HPLC Protein Purity Analysis Service

Protein purity refers to the relative proportion of the target protein in a protein sample, typically expressed as percentage. The higher the purity, the higher the proportion of the target protein in the sample. Measuring protein purity is an important aspect of biochemistry, molecular biology, and biotechnology. By determining protein purity, the quality of the extracted protein sample can be assessed, which can provide a reference for subsequent experimental procedures. High purity protein samples help ensure the reliability of experiments, especially when studying the functions, structures, and interactions of proteins. In the biopharmaceutical industry, protein purity is a critical parameter that can optimize production processes and improve product quality.

 

High-performance liquid chromatography (HPLC) is a chromatographic method used for separating, quantifying, and identifying components in complex mixtures. Since its development in the 1960s, HPLC has become the main method for determining protein purity. The principle of HPLC determination of protein purity is based on the retention time (RT) of proteins on the chromatographic column, such as reversed-phase HPLC (RP-HPLC), ion-exchange HPLC (IEX-HPLC), or size-exclusion HPLC (SEC-HPLC). HPLC uses the separation effect on the column and a highly sensitive detector to quickly and accurately measure protein purity.

 

Analysis Workflow

 



 

 

Service Advantages

1. High Precision and Sensitivity

We use advanced HPLC technology, which can accurately detect protein samples even at very low concentrations to ensure the accuracy and reliability of the results.

 

2. Wide Range of Applications

HPLC method is suitable for various types of protein samples, including natural proteins, recombinant proteins, modified proteins, etc., whether they are single proteins or protein mixtures, we can provide accurate purity analysis.

 

3. Short Experimental Cycle

Our HPLC system has high resolution and rapid analysis capabilities, allowing the detection of protein purity to be completed in a short time, meeting the time requirement of customers.



4. Professional Technical Team

We have an experienced technical team that can select the appropriate HPLC column and method according to customer needs to ensure the accuracy and reliability of the results.

 

5. Data Processing and Reporting

We provide detailed data processing and analysis reports, including chromatograms, purity percentages, etc., to help customers quickly understande and evaluate experimental results.

 

6. Customized Services

We can provide customized HPLC protein purity determination services according to customer needs, including sample treatment, column selection, method optimization, etc., to meet the needs of different experimental requirements.

 

Sample Submission Requirements

 



 

*Please use buffers suitable for HPLC analysis. Avoid using components that may adversely affect the chromatographic column, such as high concentrations of salts, chelating agents, non-volatile buffers, lipids, detergents, etc.

 

Service at MtoZ Biolabs

1. Experimental Procedures

2. Relevant Mass Spectrometry Parameters

3. Detailed Information on HPLC Determination of Protein Purity

4. Chromatographic Images

5. Raw Data