How to Use the KEGG Database to Retrieve Metabolic Pathways of a Specific Microorganism
To retrieve metabolic pathways for a specific microorganism using the KEGG database, follow these steps:
1. Navigate to the KEGG database website (https://www.kegg.jp/).
2. In the search bar, enter the name or KEGG ID of the microorganism. If the microorganism's name is unknown, try searching by its genus or other relevant identifiers.
3. Click the search button, and the system will display results based on your input.
4. On the search results page, you will see a list of strains or species related to your search. Select the microorganism strain or species of interest.
5. Click the link for the selected microorganism to access its detailed information page.
6. The detailed information page will display basic information about the microorganism, including classification, genomic details, and more.
7. In the left-hand navigation bar of the detailed page, click the "Pathway" link to access the metabolic pathway information.
8. On the metabolic pathway page, you will find graphical representations of the microorganism’s known metabolic pathways, including pathway diagrams and associated metabolic products.
9. Click on each node within the pathway diagram to view detailed information about the corresponding metabolic enzymes, substrates, and products.
10. To explore a specific metabolic pathway in greater detail, click the link in the pathway diagram to open the pathway's dedicated page.
11. The pathway’s detailed page will provide an in-depth description of the pathway, including the enzymes and substrates involved.
12. To learn more about a specific enzyme or substrate, click the relevant link to view further details on that enzyme or substrate.
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