How to Perform Proteomics Sample Pretreatment?
Proteomics sample pretreatment is a crucial procedure that has a direct impact on the quality and accuracy of protein analysis. The detailed steps for proteomics sample pretreatment are as follows:
1. Protein Concentration Determination
Quantify the extracted proteins using appropriate methods, such as the bicinchoninic acid (BCA) assay, Bradford assay, or other suitable colorimetric assays. This step ensures accurate preparation for subsequent experiments and quantitative analyses by maintaining consistent protein amounts across all samples.
2. Protein Purification
Depending on experimental requirements, purify proteins using commonly employed techniques such as SDS–PAGE gel electrophoresis, two-dimensional gel electrophoresis, or liquid chromatography (e.g., high-performance liquid chromatography, HPLC).
3. Sample Storage
Divide the processed samples into small aliquots and store them at −80 °C or lower to prevent protein degradation.
MtoZ Biolabs, an integrated chromatography and mass spectrometry (MS) services provider.
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