How to Analyze SDS-PAGE Bands for Purified GST-tagged Protein Samples?
SDS-PAGE electrophoresis is a common protein analysis method that separates proteins based on their molecular weight. For purified GST-tagged protein samples, one or more protein bands should be visible after SDS-PAGE. The following are general steps for analyzing SDS-PAGE results:
Determine Protein Band Position
First, determine the position of the GST-tagged protein on the gel. This is usually done by comparing the migration distance of the sample to a molecular weight standard. A band corresponding to the expected molecular weight of the GST-tagged protein should be visible on the gel.
Evaluate Protein Purity
If the sample is purified GST-tagged protein, there should be a single prominent band on the gel. Multiple bands may indicate the presence of contaminating proteins.
Quantify Protein
Protein quantification can be done by comparing the intensity of the sample band to that of a known concentration protein standard. Specialized software is required to analyze gel images for this purpose.
Check for Protein Degradation
If smaller bands are observed beneath the expected GST-tagged protein band, it may indicate that the protein has undergone degradation during purification or storage.
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