High Resolution Mass Spectrometry (HRMS) Analytical Service
- Drug Development: Structural identification of new drug candidates, detection of metabolites, and impurity characterization.
- Bioanalysis: Research on protein modifications, metabolomic compounds, and small-molecule biomarkers.
- Environmental Science: Detection and qualitative analysis of pollutants, persistent organic compounds, and trace toxins.
- Food Testing: Detection and quantification of food additives, contaminants, and natural components.
High Resolution Mass Spectrometry (HRMS) Analytical Service is a molecular mass and structural characterization service based on high-resolution mass spectrometry, which can distinguish extremely small mass differences in complex samples and provide highly accurate mass spectrometry data. With sub-ppm mass accuracy and excellent resolution, HRMS can differentiate isotopes, isomers, and trace impurities in complex matrices, making it a core tool in drug development, bioanalysis, environmental science, and food safety testing.
Technical Principles
The core principle of HRMS lies in measuring the mass-to-charge ratio (m/z) of ions with extremely high resolution and mass accuracy. During analysis, sample molecules are first converted into charged ions using techniques such as electrospray ionization (ESI), atmospheric pressure chemical ionization (APCI), or matrix-assisted laser desorption ionization (MALDI). These ions are then accelerated and separated according to their mass-to-charge ratio in high-resolution mass spectrometry platforms such as Orbitrap or time-of-flight (TOF) mass spectrometers. The detector captures the signals and generates high-resolution mass spectra, enabling the precise differentiation of compounds with only subtle mass differences. Isotopic distribution, fragment ion information, and accurate mass data are further applied to molecular formula determination and structural elucidation, giving HRMS an irreplaceable advantage in complex sample analysis and trace detection.
Analysis Workflow
The general process of High Resolution Mass Spectrometry (HRMS) Analytical Service is as follows:
1. Sample Preparation
Perform appropriate dilution or pretreatment according to the sample properties to ensure compatibility with injection and ionization requirements.
2. Sample Detection
Use suitable ionization techniques such as ESI, APCI, or MALDI to convert target molecules into charged ions, and separate and detect them on high-resolution mass spectrometry platforms such as Orbitrap or TOF to obtain high-resolution mass spectra.
3. Data Analysis
Analyze the mass spectrometry data with specialized software, calculate molecular masses with database support, deduce molecular formulas, and perform component identification.
4. Report Generation
Provide a complete report that includes experimental procedures, mass spectra, and data analysis.
Kvivik I. et al. Pharmaceuticals (Basel). 2021.
Figure 1. Mass Spectrum from High Resolution Mass Spectrometry (HRMS), Identifying High Mobility Group Box 1 (HMGB1) Peptide IKGEHPGLSIGDVAK
Service Advantages
1. High Resolution and Accuracy
Capable of distinguishing subtle mass differences with sub-ppm mass accuracy.
2. Multiple Ionization and Detection Platforms
Supports ionization techniques such as ESI, APCI, and MALDI, and is equipped with advanced mass spectrometry systems, including Orbitrap and TOF, to meet a wide range of application needs.
3. Complex Matrix Analysis Capability
High Resolution Mass Spectrometry (HRMS) Analytical Service enables accurate detection and identification of trace components in complex matrices such as pharmaceutical, environmental, food, or biological samples.
4. Structural Elucidation Support
By combining accurate mass, isotopic distribution, and fragment ion information, HRMS can not only determine molecular formulas but also assist in elucidating compound structures.
Services at MtoZ Biolabs
Using high-resolution mass spectrometry and multiple ionization techniques, MtoZ Biolabs provides High Resolution Mass Spectrometry (HRMS) Analytical Service for unknown compound identification, molecular formula confirmation, and impurity structure elucidation. We select the optimal ionization mode and analytical strategy according to sample properties, and combine advanced systems such as Orbitrap and TOF to ensure results with high resolution and mass accuracy. The final mass spectra and data reports we deliver not only meet the needs of scientific research but also support compliance requirements in drug development and quality control.
Sample Submission Suggestions
Please provide samples with the highest possible purity, and if the sample is in a complex matrix, include background information or pretreatment details. It is recommended to contact our technical support team prior to submission to assess sample suitability and obtain tailored submission guidance.
Applications
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