High-Depth Proteomics Service

Proteins serve as key functional molecules in biological phenotypes and disease development, making their study essential for understanding biological mechanisms and developing novel therapies. However, traditional proteomics methods face significant limitations: techniques based on 2D gel electrophoresis typically identify around 1,000 proteins, while conventional enzymatic peptide analyses can detect a maximum of 3,000 to 4,000 proteins. This coverage represents only about 5% to 20% of the total proteome, which is insufficient for the demands of high-throughput quantitative analysis.

 

In response to these challenges, high-throughput, high-coverage proteomics technologies have emerged. This technology combines advanced mass spectrometry (MS) techniques with refined sample preparation, enabling efficient and accurate quantification and identification of thousands of proteins in complex biological samples. By utilizing labeling techniques such as isobaric tags for relative and absolute quantitation (iTRAQ) and stable isotope labeling with amino acids in cell culture (SILAC), researchers can achieve enhanced sensitivity and greater depth of analysis, particularly in detecting low-abundance proteins.

 

MtoZ Biolabs is committed to providing High-Depth Proteomics Service, catering to the increasing demands in various research areas, including biomarker discovery, disease mechanism exploration, and personalized medicine. Our comprehensive services enable researchers to gain insights into protein dynamics and interactions, facilitating advancements in scientific knowledge and therapeutic applications.

 

Analysis Workflow

 



 

Service Advantages

1. High Sensitivity

Capable of detecting low-abundance proteins, thereby enhancing the analytical capability of complex biological samples.

 

2. High Throughput

Allows for simultaneous analysis of multiple samples, significantly enhancing experimental throughput.

 

3. Strong Accuracy

Integrates various quantitative techniques to ensure reliable and reproducible results.

 

4. Comprehensiveness

Offers broad coverage, making it suitable for a diverse range of biological sample types.

 

Sample Submission Requirements

 



For more sample details, please consult our technical team.

 

Applications

• Identifying early disease markers through the analysis of characteristic proteins present in blood samples.
• Investigating the impact of drugs on protein expression to better understand their mechanisms of action.
• Supporting the discovery of novel biomarkers to advance clinical applications and translational research.
• Conducting detailed analyses of protein changes within the tumor microenvironment, thereby aiding the development of innovative therapeutic strategies.