High-Depth Blood Proteomics Service
- High-Throughput Detection: Capable of identifying and quantifying thousands of proteins in a single analysis, catering to large-scale sample analysis.
- High Sensitivity: Effectively detects low-abundance biomarkers, enabling early disease diagnosis.
- Rapid Delivery: Short analysis cycles enhance experimental efficiency and reduce research time.
- Data Reliability: Standardized protocols and advanced analytical methods ensure repeatability and reliability of results.
- Hemolysis must be avoided during sample preparation.
- Plasma samples should be utilized within three months, with fresher samples preferred.
- The minimum submission volume is 100 μL.
- Biomarker Discovery: Identifying disease-related proteins to support early diagnosis and clinical applications.
- Drug Mechanism Research: Analyzing the effects of drugs on the protein landscape, aiding in new drug development and evaluation.
- Disease Mechanism Exploration: Investigating protein expression changes in various disease states to uncover potential biological mechanisms.
- Basic Research: Providing substantial data support for cell and molecular biology research, advancing scientific understanding.
Blood is an essential biological specimen in clinical research, playing a crucial role in early disease diagnosis, prognostic evaluation, and personalized treatment. It contains a diverse array of protein molecules that sustain physiological functions and reflect the health status of the organism during disease. In recent years, the rapid advancement of proteomics technology has led researchers to increasingly focus on blood protein groups, with the goal of discovering new biomarkers that facilitate early diagnosis and precise treatment of diseases.
Zhao, Y. et al. J. Proteome Res. 2023.
Figure 1. Evolution of Mass Spectrometry Instruments and Techniques for Blood Proteomics
However, blood proteomics currently faces several challenges. For instance, high-abundance proteins in plasma can obscure the information from low-abundance proteins, leading to biased analysis results. Additionally, traditional mass spectrometry techniques often exhibit limited sensitivity when analyzing proteins across a wide dynamic range. To tackle these issues, MtoZ Biolabs has launched the High-Depth Blood Proteomics Service, integrating an automated flexible sample processing platform with the innovative Orbitrap Astral mass spectrometer. This service provides a comprehensive analysis of the complex protein groups in blood samples, characterized by high sensitivity and throughput, thereby catering to the diverse needs of researchers at various stages of their studies. The Service delivers robust data support for investigating disease mechanisms by thoroughly analyzing the variations of thousands of proteins in blood.
Analysis Workflow
1. Sample Collection and Processing
Blood samples are collected using EDTA anticoagulant tubes, gently mixed, and centrifuged at 4°C for 10 minutes to separate the plasma.
2. Protein Extraction and Purification
The upper plasma layer is transferred to a centrifuge tube, protease inhibitors are added, and the sample is rapidly frozen in liquid nitrogen to maintain quality.
3. Enzymatic Digestion and Peptide Generation
The extracted proteins are digested to produce peptides suitable for mass spectrometric analysis.
4. Mass Spectrometry Analysis
High-throughput mass spectrometry devices conduct LC-MS/MS analysis to comprehensively capture protein data.
5. Data Processing and Analysis
Advanced software, along with bioinformatics techniques, is employed for precise protein identification and quantification.
Service Advantages
Sample Submission Requirements
1. Sample Type: Blood samples are not restricted by species.
2. Sample Collection: Blood is collected using EDTA anticoagulant tubes (purple cap), gently mixed, and centrifuged at 4°C.
3. Sample Preservation: The upper plasma layer is transferred to a centrifuge tube, protease inhibitors are added, and the sample is rapidly frozen in liquid nitrogen, stored at -80°C.
Notes:
Applications
MtoZ Biolabs is dedicated to delivering professional High-Depth Blood Proteomics Service, assisting researchers in exploring blood protein information to achieve breakthroughs and innovations in scientific inquiry. Our team is committed to offering high-quality services and support.
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