Gel Permeation Chromatography (GPC) Analytical Service
- Pharmaceutical Industry: Evaluation of molecular weight and homogeneity of pharmaceutical excipients and polymer carriers.
- Polymer Materials Research: Quality control and performance studies of synthetic polymers, composites, and coatings.
- Biomacromolecule Characterization: Molecular weight and homogeneity testing of natural biomacromolecules such as proteins, polysaccharides, and nucleic acids.
- Food and Cosmetics: Molecular weight characterization of thickeners, stabilizers, and emulsifiers in complex formulations.
Gel Permeation Chromatography (GPC) Analytical Service is a polymer analysis service that separates and characterizes polymers based on size differences. As a type of size exclusion chromatography (SEC), GPC provides key parameters such as number-average molecular weight (Mn), weight-average molecular weight (Mw), and polydispersity index (PDI), providing researchers with a deeper understanding of the relationship between material structure and performance. With the widespread application of polymers in drug delivery systems, biomedical materials, functional coatings, and high-performance polymers, this service has become a crucial tool for quality control, formulation optimization, and functional evaluation.
Technical Principles
The basic principle of GPC is that the porous stationary phase filler generates a separation effect on molecules of different sizes. Large molecules elute earlier because they cannot enter the pores, while small molecules enter the pores and are eluted later, thereby achieving separation based on molecular size. By combining high-sensitivity detectors such as refractive index detectors, ultraviolet detectors, or multi-angle light scattering detectors, it is possible to obtain the molecular weight distribution curve, degree of polymerization information, and component content of the sample, which helps researchers gain a deeper understanding of the molecular structure characteristics of materials.
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Figure 1. Schematic Diagram of Gel Permeation Chromatography (GPC) System
Analysis Workflow
The general process of Gel Permeation Chromatography (GPC) Analytical Service is as follows:
1. Sample Pretreatment
Dissolve, dilute, and filter the sample according to its type to ensure uniformity and compatibility with the analysis.
2. Chromatographic Separation
Achieve separation of molecules based on size within the GPC system.
3. Signal Detection
Record elution curves using detectors such as refractive index (RI), ultraviolet (UV), or light scattering.
4. Data Analysis
Calculate molecular weight parameters and distribution curves through calibration with standards or by using light scattering data.
5. Report Generation
Deliver a complete chromatogram, molecular weight parameters, and distribution curves, along with a standardized analytical report.
Service Advantages
1. High-Precision Molecular Weight Determination
Provides accurate number-average molecular weight (Mn), weight-average molecular weight (Mw), and polydispersity index (PDI), enabling comprehensive characterization of polymer molecular weight and distribution features.
2. Multiple Detector Combination
Equipped with refractive index (RI), ultraviolet (UV), and multi-angle light scattering (MALS) detectors, enabling both relative and absolute molecular weight determination and ensuring data reliability.
3. Broad Sample Applicability
Gel Permeation Chromatography (GPC) Analytical Service can be applied to molecular weight characterization of polymers, biomacromolecules, excipients in injections, and other types of samples.
4. Customized Solutions
Flexibly adjusts testing conditions and solvent systems according to client objectives to meet diverse research needs.
5. Comprehensive Data Support
Provides not only molecular weight parameters but also distribution curves, residual analysis, and component content.
Services at MtoZ Biolabs
Relying on gel permeation chromatography platforms and multiple types of detectors, MtoZ Biolabs provides Gel Permeation Chromatography (GPC) Analytical Service for molecular weight and distribution characterization of polymers, biomacromolecules, excipients in injections, and other samples. We flexibly select mobile phases and detection modes according to sample characteristics and research needs, and deliver complete chromatograms, molecular weight parameters, and distribution curves to support clients in obtaining reliable data for research or production.
Sample Submission Suggestions
Please ensure that samples are soluble in the designated mobile phase. For samples with poor solubility, clients are advised to dissolve them in advance to obtain a clear and homogeneous solution. The dissolved samples should be filtered through membranes with appropriate pore sizes to prevent blockage of the chromatography system.
It is recommended to contact our technical support team before submission to assess sample suitability and obtain tailored submission guidance.
Applications
Related Services
Gel Permeation Chromatography for Molecular Weight Determination Service
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