Free Amino Acid Analysis Service
- Comprehensive Coverage: Analysis of over 100 free amino acids, including rare and commonly studied variants, enables a complete metabolic overview.
- Advanced Accuracy: High sensitivity and resolution are achieved through the integration of HPLC and MS, ensuring precise results for even complex samples.
- Customizable Solutions: Flexible sample preparation options and tailored reporting formats cater to diverse research needs.
- Integrated Insights: Beyond raw data, we provide contextualized interpretations, linking amino acids to their metabolic pathways and potential biological significance.
- One-Time-Charge: Our pricing is transparent, no hidden fees or additional costs.
Free amino acid analysis is a technique for the quantitative and qualitative measurement of free amino acids in various samples. Free amino acids, defined as those not bonded via peptide bonds, play crucial roles in cellular metabolism, energy synthesis, neurotransmitter regulation, and diverse physiological processes. Compared to total amino acids, free amino acids exhibit greater flexibility in participating in physiological activities, making them particularly important in biomedical research, nutrition science, and disease diagnostics. Accurate free amino acid analysis allows researchers to gain a deeper understanding of metabolic networks, identify disease-related biomarkers, and evaluate nutritional status under specific conditions.
Services at MtoZ Biolabs
MtoZ Biolabs offers professional Free Amino Acid Analysis Service enabling clients to identify and quantify over 100 free amino acids in their samples. Leveraging cutting-edge High-Performance Liquid Chromatography (HPLC) and Mass Spectrometry (MS) technologies, we deliver results with exceptional sensitivity and resolution. Unlike conventional methods that focus on the 20 standard amino acids, our services provide an extended amino acid profile, offering a broader perspective on metabolic processes.
Analysis Workflow
To ensure accurate and reliable results, MtoZ Biolabs' Free Amino Acid Analysis Service follows a streamlined workflow designed to maintain sample integrity and deliver precise data.
1. Sample Preparation
Our tailored guidelines for processing and preserving samples, such as serum, urine, and cell culture media, ensure the stability and integrity of free amino acids throughout the analysis.
2. Extraction and Separation
Using advanced techniques, we effectively isolate free amino acids, removing interfering substances while preserving their structural integrity.
3. Liquid Chromatography
Precise separation of amino acids is achieved through state-of-the-art liquid chromatography methods, ensuring high-resolution results.
4. Mass Spectrometry
Advanced mass spectrometry provides accurate qualitative and quantitative results, generating detailed amino acid profiles.
5. Data Analysis and Reporting
Detailed reports include the precise concentrations of each free amino acid, along with relevant statistical insights. Customized interpretations and reporting formats are available upon request to meet specific research or project needs.
Service Advantages
Case Study
Case 1: Free Amino Acids Analysis Reveals the Influence of Tea Varieties and Processing Methods on Composition
This study utilized HPLC-MS/MS to analyze free amino acid content in different tea varieties (e.g., green tea, black tea) and assessed changes induced by black tea processing. Significant differences in amino acid profiles were observed between tea types, with key amino acids undergoing substantial transformations during processing, influencing flavor and functional properties. These findings underscore the importance of Free Amino Acid Analysis Service in nutritional studies and processing optimization, providing scientific support for quality control and functional food development.
Yılmaz, C. et al. LWT. 2019.
Case 2: Biogenic Tracing of Atmospheric Aerosols Through Free Amino Acid Analysis
Using GC-MS and UPLC-MS, this study quantified free amino acids and sugars in plant pollens, revealing their chemical characteristics and contributions to atmospheric aerosols. Free amino acids accounted for 0.29% to 15% of dry weight, with proline as the dominant component, while sugars ranged from 4.0% to 29%. The chemical profile of pine pollen closely matched aerosols from the Lake Tahoe region, illustrating the utility of pollen components in tracing aerosol sources. This research demonstrates the value of Free Amino Acid Analysis Service in environmental studies and atmospheric research.
Axelrod, K. et al. Sci. Total Environ. 2021.
MtoZ Biolabs, a provider of advanced Chromatography and Mass Spectrometry (MS) solutions, is dedicated to delivering high-quality Free Amino Acid Analysis Service to researchers in biochemistry, biotechnology, and biopharmaceutical fields. With our expertise in proteomics and metabolomics, we strive to provide rapid, high-throughput, and cost-effective analysis tailored to your specific needs. Contact us today for a free project evaluation and discover how our Free Amino Acid Analysis Service can accelerate your research and unlock deeper metabolic insights!
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