Immune Regulation Related Factors Analysis Service

To maintain the immune system's homeostasis, multiple control systems are essential for managing infections, inhibiting tumor growth, preventing inflammation, and avoiding autoimmune diseases. T cells are known to be critical in this regulatory process. Specifically, Th3 cells primarily secrete TGF-β along with varying levels of IL-4 and IL-10. Natural regulatory T cells and Tr1 cells can suppress cytokine production in vitro through CD4+/CD25- T cells or antigen-specific Th1 or Th2 cells. Other T cell types, such as Th1 cells, produce INF-γ, and nkT cells are noted for their ability to release regulatory cytokines including IL-4, IL-10, and IL-13.

MtoZ Biolabs employs flow cytometry to quantitatively analyze cytokines and proteins involved in immune regulation. This technique offers enhanced sensitivity and a wider range of detection. The following table lists cytokines detectable in human samples, with services also available for other species.

Immunomodulation-Related Factor Detection— Flow Cytometry

Figure 2. Detection of Immunomodulation-Related Factors by Flow Cytometry

Sample Submission Requirements

1. A Minimum of 25 µL of Sample is Required, Compared to the 50 µL or 100 µL Needed for Conventional ELISA

2. Applicable to Serum, Plasma, Cell Culture Supernatants, and Various Bodily Fluids

3. Bodily Fluid Samples must be Stored at -80 ℃ and Shipped with Dry Ice

Deliverables

In the technical report, MtoZ Biolabs will provide you with detailed technical information, including: 

1. Experimental Procedures

2. Raw Data (Fluorescence luminescence Spectrum)

3. Technical Report

4. Absolute Content of Each Detection Indicator (Excel Spreadsheet)