Exploring the Application and Breakthroughs of New Technologies in Protein Purity Identification

The application and breakthrough of new technologies in protein purity identification bring broad prospects for biological research and clinical applications. By introducing more accurate, high-throughput, and high-sensitivity new technologies, we can better evaluate protein purity and ensure the quality and safety of biological products.

 

Protein purity is one of the essential indicators of the quality of biological products, and is crucial for ensuring the safety and efficacy of drugs. In recent years, with the continuous progress of science and technology, new technologies have been widely used in the field of protein purity identification, achieving breakthroughs in improving the accuracy and efficiency of identification.

 

Limitations of Traditional Protein Purity Identification Technologies

Traditional protein purity identification techniques such as gel electrophoresis and high-performance liquid chromatography have been widely used, but there are still some limitations. For example, these methods may be interfered by sample complexity, low concentration samples, and isomers, leading to limitations in accuracy and sensitivity.

 

Application and Breakthrough of New Technologies in Protein Purity Identification

1. Mass Spectrometry

The application of mass spectrometry in protein purity identification has developed rapidly. With high-resolution mass spectrometers and bioinformatics analysis methods, the molecular weight, amino acid sequence, and glycosylation of proteins can be accurately measured, realizing the accurate identification of protein purity.

 

2. Biosensor Technology

Biosensor technology uses the specific interaction between proteins and ligands to achieve rapid quantification and purity identification of proteins. For example, technologies such as Surface Plasmon Resonance (SPR) and Bio-Layer Interferometry can monitor the binding reaction of proteins and ligands in real time, and quickly obtain purity information.

 

3. Mass Spectrometry

Mass spectrometry combines mass spectrometry and optical technology to measure the absorption and scattering signals of proteins in the spectrum range, realizing the quantitative determination of protein purity. This method has the advantages of high throughput, high sensitivity, and rapid analysis, and is suitable for the purity identification of large-scale samples.

 

Prospects of New Technologies in Biological Research and Clinical Applications

The application of new technologies in protein purity identification brings many possibilities for the development and quality control of biological products. First, accurate protein purity identification can improve the quality and safety of biological products, ensuring the efficacy and stability of drugs. Secondly, new technologies can effectively solve the limitations of traditional methods, improving the accuracy, sensitivity, and high-throughput analysis capability of identification. In addition, new technologies can be applied to the discovery of disease markers, drug research and development, and personalized medicine, providing better support for clinical diagnosis and treatment.

 

Application Cases

Significant progress has been made in the identification of protein purity using new technologies. For example, mass spectrometry has been widely used in the research and development and quality control of biological products, effectively improving the accuracy and sensitivity of identification. Biosensor technology plays an important role in drug screening and clinical diagnosis. Mass spectrometry shows great potential in quality control of biological products and batch analysis.