Exosome Supernatant Preparation Service
- Assess sample type, volume, and specific research requirements
- Remove large cellular debris through low-speed centrifugation
- Optimize pre-processing based on sample complexity
- Apply ultracentrifugation, size-exclusion chromatography (SEC), or polymer precipitation
- Remove contaminants and free proteins using filtration or density gradient purification
- Obtain high-purity exosome supernatant with minimal non-exosomal content
- Assess exosome size and concentration using nanoparticle tracking analysis (NTA) or dynamic light scattering (DLS)
- Verify sample purity via Western blot (CD63, CD81, CD9 markers) or electron microscopy
- Ensure RNA integrity for downstream transcriptomic analysis
- Aliquot and store samples under optimal conditions to maintain exosome integrity
- Deliver prepared exosome supernatants ready for proteomics, RNA sequencing, and therapeutic applications
Exosomes are nanoscale extracellular vesicles (30–150 nm in diameter) secreted by nearly all cell types. They play a crucial role in intercellular communication by transferring proteins, lipids, and genetic material, thereby influencing various biological processes such as immune regulation, tissue repair, and disease progression. Due to their ability to serve as biomarkers and therapeutic agents, exosomes are extensively studied in disease diagnosis, drug delivery, and regenerative medicine. Exosomes can be found in various biological samples, including:
Body fluids: Blood (plasma/serum), urine, cerebrospinal fluid (CSF), saliva, amniotic fluid, and breast milk
Cell culture media: Conditioned media from different cell lines used in research and therapeutic applications
Tissue samples: Solid tissues where exosomes can be extracted post-homogenization
Gurung, S. et al. Cell, Commun, Signal. 2021.
Figure 1. The Structure of Exosome
The isolation of exosomes from these samples requires a precise and efficient workflow to ensure high purity and integrity. Common isolation methods include ultracentrifugation, size-exclusion chromatography (SEC), polymer-based precipitation, ultrafiltration, and microfluidic approaches. However, raw samples often contain cell debris, proteins, and other contaminants, making exosome supernatant preparation a critical step before downstream analysis. The exosome supernatant preparation process ensures that exosomes are effectively separated from other unwanted components, improving sample clarity and reliability for applications such as proteomics, RNA analysis, and functional studies. High-quality exosome supernatants enable more accurate biomarker discovery, therapeutic research, and drug development.
Chen, J. et al. Front, Bioeng, Biotechnol. 2022.
Figure 2. The Workflow of Exosome Isolation
Services at MtoZ Biolabs
At MtoZ Biolabs, we provide high-quality Exosome Supernatant Preparation Service to ensure the efficient isolation of exosome-rich fractions while removing contaminants that may interfere with downstream analysis. Our service is designed for researchers and biotech companies seeking high-purity exosomes from various sample types for applications such as diagnostics, regenerative medicine, and drug discovery. We employ optimized protocols using ultracentrifugation, filtration, precipitation, and chromatography-based methods to yield high-quality exosome supernatants tailored to specific experimental needs. Our standardized yet customizable workflow ensures high recovery, minimal loss, and reproducible results, making our service ideal for biomarker analysis, functional assays, and therapeutic research.
Analysis Workflow
1. Sample Evaluation & Pre-Treatment
2. Exosome Isolation & Supernatant Preparation
3. Quality Control & Sample Integrity Assessment
4. Final Sample Processing & Storage
Service Advantages
✅ High Purity & Yield – Optimized protocols ensure minimal contaminants and efficient exosome recovery
✅ Customizable Workflows – Tailored protocols based on sample type and downstream application
✅ Advanced Isolation Techniques – Combination of ultracentrifugation, filtration, and chromatography for best results
✅ Quality-Controlled Outputs – Verified exosome integrity using DLS, NTA, and protein marker validation
✅ Fast Turnaround Time – Reliable and reproducible results delivered within industry-standard timeframes
Applications
✔ Biomarker Discovery – Enabling early disease diagnosis and personalized medicine research
✔ Regenerative Medicine – Facilitating stem cell-derived exosome research for tissue repair and therapy
✔ Cancer Research – Investigating exosome-mediated tumor microenvironment interactions and drug resistance
✔ Neurodegenerative Diseases – Studying the role of exosomes in Alzheimer’s, Parkinson’s, and other CNS disorders
✔ Drug Delivery & Therapeutics – Developing exosome-based drug carriers for targeted therapy
Deliverables
1. High-purity exosome supernatant free of non-exosomal contaminants
2. Fully characterized exosome fractions with size, concentration, and protein marker validation
3. Ready-to-use exosome supernatants for proteomics, transcriptomics, and functional assays
4. Customizable reporting with detailed isolation methods and quality assessment data
The successful isolation and purification of exosome supernatants are crucial for advancing exosome-based research and clinical applications. MtoZ Biolabs is dedicated to delivering high-quality, reproducible, and contamination-free exosome supernatants, supporting researchers in biomarker discovery, drug development, and therapeutic innovation. With our state-of-the-art isolation technologies, stringent quality control measures, and customizable workflows, we provide tailored solutions for your exosome research needs. Contact MtoZ Biolabs today to discuss your project and receive expert guidance on exosome supernatant preparation!
How to order?
