Exosome - NGS Services (RNA Next Generation Sequencing)

Exosome - NGS Services (RNA Next Generation Sequencing) is a service based on next-generation sequencing (NGS) technology, designed to sequence and analyze the expression of various RNAs in exosomes, supporting various applications such as disease mechanism research, biomarker screening, and new drug development. Exosomes are widely present in biological fluids such as blood, urine, cerebrospinal fluid, and saliva, carrying various biomolecules including proteins, RNA, and DNA. These biomolecules are delivered via exosomes to mediate long-distance gene regulation, metabolic remodeling, and pathological processes. Comprehensive analysis of the exosomal RNA profile and understanding its dynamic changes in diseases and physiological states can help reveal molecular mechanisms of diseases and discover non-invasive biomarkers. Due to the complexity and low abundance of exosomal RNA, traditional detection methods struggle to fully analyze the exosomal RNA landscape. High-throughput sequencing based on RNA provides a powerful solution for exosomal nucleic acid research. Utilizing NGS technology, MtoZ Biolabs offers Exosome - NGS Services (RNA Next Generation Sequencing) to help researchers systematically, deeply, and accurately explore the value of exosomal RNA.

 



Elkommos-Zakhary M. et al. Non-Coding RNA. 2022.

Figure 1. Schematic representation detailing the discovery of RNA biomarkers from exosomes.

 

Analysis Workflow

1. Sample Preparation and Exosome Isolation

Choose the appropriate method to extract high-purity exosomes and validate their quality.

 

2. RNA Extraction

Use a dedicated extraction kit optimized for low-abundance RNA from exosomes to extract total RNA.

 

3. NGS Library Construction

Construct different sequencing libraries based on the target RNA types.

 

4. High-Throughput Sequencing

Perform single-end or paired-end sequencing using the Illumina platform.

 

5. Data Analysis and Report

Conduct data analysis and bioinformatics analysis, providing a comprehensive data report.

 

Applications

Here are examples of applications for Exosome - NGS Services:

Disease Biomarker Screening

Systematically screen exosomal RNA to identify non-invasive diagnostic and prognostic molecules associated with diseases.

 

Disease Molecular Mechanism and Cell Communication Research

Conduct a comprehensive analysis of the exosomal RNA profile to explore its role in intercellular communication, signal transduction, immune regulation, tumor microenvironment, and other processes.

 

Discovery of Novel RNA Molecules

Identify novel miRNA, lncRNA, and circRNA in exosomes that are not yet annotated, expanding the understanding of exosome function and uncovering new therapeutic targets.

 

Services at MtoZ Biolabs

Leveraging advanced technology platforms and a team of experienced experts, MtoZ Biolabs offers Exosome - NGS Services to support one-stop analysis of exosomal mRNA, miRNA, lncRNA, circRNA, and the whole transcriptome. This service helps researchers systematically analyze the exosomal RNA profile and uncover potential biomarkers and molecular mechanisms.

MtoZ Biolabs, an integrated Chromatography and Mass Spectrometry (MS) Services Provider, provides advanced proteomics, metabolomics, and biopharmaceutical analysis services to researchers in biochemistry, biotechnology, and biopharmaceutical fields. Our ultimate aim is to provide more rapid, high-throughput, and cost-effective analysis, with exceptional data quality and minimal sample consumption.

 

FAQ

Q. How to Ensure the High Purity of Isolated Exosomes and Avoid Contamination by Cell Debris or Free RNA?

To ensure the high purity of exosomes and avoid contamination by cell debris or free RNA, we use a multi-step combined isolation strategy. This typically involves a combination of ultracentrifugation (or SEC) and immunoaffinity enrichment (targeting CD9/CD63/CD81) to extract exosomes from bodily fluids or cell culture supernatants. And all isolated samples undergo strict quality control. In addition, before RNA extraction, we treat the samples with free nucleases (such as RNase) to ensure that the RNA extracted is genuinely encapsulated within the exosomes, maximizing the scientificity and reliability of the results.

 

Q. How are the Data Generated by Exosome - NGS services Interpreted and Analyzed?

Exosome-NGS data analysis involves bioinformatics processing to identify and quantify the exosomal RNA molecules. Researchers can analyze differential expression, pathway enrichment, and network interactions to uncover the biological significance of exosomal contents and their potential roles in health and disease.

 

Case Study

This study employed NGS technology to comprehensively analyze exosomal microRNAs (miRNAs) isolated from the plasma of patients with acute type A aortic dissection accompanied by acute lung injury (ATAAD-ALI). A series of miRNAs were systematically identified as significantly differentially expressed in ATAAD-ALI patients, with some miRNAs showing marked upregulation or downregulation compared to patients with ATAAD alone and healthy individuals. Further bioinformatics analysis revealed that these dysregulated miRNAs are primarily involved in pathways related to inflammation, apoptosis, immune regulation, and lung injury.

 



Zhang C. et al. Sci Rep. 2022.

Figure 2. Schematic overview of the strategy for identifying an plasma exosomal miRNAs signature associated with ATAAD with ALI.