Exosomal circRNA Sequencing Service

Exosomal circRNA Sequencing Service utilizes high-throughput sequencing technology for comprehensive detection and analysis of exosomal circular RNA (circRNA), providing essential data support for both basic research and clinical translation. Circular RNAs (circRNAs) are a class of non-coding RNAs with a stable covalently closed-loop structure, playing crucial roles in gene regulation, cell signaling, and disease development. In recent years, exosomes, as key mediators of intercellular communication, have been found to be enriched with circRNAs, which can exert regulatory functions in both local and distant target cells. For instance, exosomal circRNAs can promote or inhibit cell proliferation, support tumor cell survival, invasion, and metastasis. A comprehensive analysis of exosomal circRNAs is instrumental in advancing disease research and precision medicine. Leveraging high-throughput sequencing technology, MtoZ Biolabs offers Exosomal circRNA Sequencing Service to enable an in-depth analysis of exosomal circRNA expression profiles, uncover regulatory networks, and reveal their roles in cell communication, disease mechanisms, and biomarker discovery.

 



Han Z. et al. Front Oncol. 2022.

Figure 1. Main functions of circRNAs.

 

Analysis Workflow

1. Exosome Isolation

High-purity exosomes are isolated using ultracentrifugation, immunomagnetic beads, or size-exclusion chromatography (SEC).

 

2. RNA Extraction

Total RNA is extracted from exosomes using optimized reagent kits.

 

3. Linear RNA Removal

RNase R digestion is used to degrade linear RNAs, ensuring only circular RNAs remain.

 

4. Library Construction

Construction of circRNA-specific sequencing libraries.

 

5. High-Throughput Sequencing

High-depth sequencing is performed using platforms such as Illumina.

 

6. Data Analysis and Reporting

Data processing and bioinformatics analysis are conducted, with a detailed report provided.

 

Service Advantages

1. Advanced Analysis Platform: MtoZ Biolabs established an advanced Exosomal circRNA Sequencing Service platform, guaranteeing reliable, fast, and highly accurate analysis service.

2. One-Time-Charge: Our pricing is transparent, no hidden fees or additional costs.

3. High-Data-Quality: Deep data coverage with strict data quality control. AI-powered bioinformatics platform integrates all Exosomal circRNA Sequencing Service data, providing clients with a comprehensive data report.

4. Accurate Detection of Low-Abundance circRNAs: RNase R treatment and specialized library construction effectively remove linear RNAs, ensuring highly specific detection of circRNAs.

 

Applications

Biomarker Discovery

Identification of exosomal circular RNAs associated with disease states or physiological conditions.

 

Functional Mechanism Studies

Investigation of the roles of exosomal circular RNAs in cell communication and disease progression.

 

Drug Development

Exploration of exosomal circular RNAs as potential targets for therapeutic and targeted interventions.

 

FAQ

Q. Is Data Analysis Provided? What does it Include?

Yes, MtoZ Biolabs offer comprehensive bioinformatics analysis, covering circRNA identification, quantitative analysis, functional prediction, and regulatory network construction, ensuring the scientific integrity and interpretability of research data.

 

Q. Why Choose Exosomal circRNA Sequencing?

Exosomal circRNA Sequencing provides a comprehensive profile of circRNAs within exosomes, serving as a cutting-edge tool for exploring cell communication, disease mechanisms, and biomarker discovery. It offers high stability and specificity and is widely applied in cancer, neurological disorders, cardiovascular diseases, and immune regulation research.

 

Case Study

This study utilized Exosomal circRNA Sequencing technology to analyze the exosomal circRNA expression profile in a mouse model of intestinal ischemia-reperfusion (I/R) injury. The results revealed that circEZH2_005 was significantly downregulated in the I/R group and alleviated intestinal inflammation and cell apoptosis by regulating the Gprc5a signaling pathway. These findings suggest that circEZH2_005 may serve as a potential biomarker for intestinal I/R injury, and targeting the circEZH2_005/hnRNPA1/Gprc5a axis could be a promising therapeutic strategy for intestinal I/R injury.

 



Zhang W. et al. Nature Communications. 2023.

Figure 2. Identification and screening of exosomal circRNAs that are associated with intestinal injury in intestinal I/R mode mice.