Equol Analysis Service

Equol is a phytoestrogen and a metabolite of isoflavones, classified chemically as a non-steroidal estrogen. Its molecular formula is C₁₅H₁₄O₃, with a molecular weight of 242.27 g/mol. The structure includes an aromatic ring system with a diphenol structure, enabling equol to mimic estrogenic activity. Equol is not directly found in food but is produced by the metabolism of isoflavones by gut microbiota, particularly from daidzein found in soy. The biological activity of equol is primarily related to its estrogen-like effects, as well as its antioxidant and anti-inflammatory properties.

 

By measuring equol levels in blood or urine, the effectiveness of equol supplementation or dietary interventions (such as soy product intake) in alleviating menopausal symptoms can be evaluated, allowing for optimized personalized treatment plans. Equol and its metabolites exhibit antioxidant and anti-inflammatory effects, influencing oxidative stress and inflammatory responses in the body. Analyzing these metabolites helps assess the impact of equol on oxidative stress and inflammation.

 



Figure 1. The Structure of Equol

 

MtoZ Biolabs offers a state-of-the-art Equol Analysis Service, backed by our advanced liquid chromatography-mass spectrometry (LC-MS) platform and professional metabolomics team. Our service delivers a comprehensive equol analysis, Better understanding individual differences provides a scientific basis for personalized nutritional interventions and clinical treatments.

 

Analysis Workflow

 



 

Service Advantages

1. High Sensitivity and Accuracy

MtoZ Biolabs' liquid chromatography-mass spectrometry (LC-MS) analysis platform can detect extremely low concentrations of RA and its metabolites, which is particularly important for studying their minute changes in cells, tissues, and bodily fluids.  

 

2. High Specificity

By optimizing chromatographic separation conditions and mass spectrometry detection parameters, RA and its various metabolites can be effectively distinguished in complex biological samples, minimizing interference from other endogenous or exogenous substances and improving detection specificity.  

 

3. Customized Analysis

Tailored analytical solutions can be designed according to research needs, providing personalized solutions for different biological samples and target molecules.

 

Applications

• Uncovering new disease biomarkers
• Improving disease diagnosis and prognosis
• Accelerating the discovery of innovative therapeutics
• Elucidating new insights into disease mechanisms

 

Sample Submission Requirements

1. Sample Types

Serum, plasma, urine, tissues and other biological samples. It is recommended to select more than 3 samples with the same condition for each experiment.

 

2. Sample Volume

• Animal tissue ≥ 100 mg
• Plant tissue ≥ 200 mg
• Serum/ Plasma ≥ 200 μL
• Urine ≥ 2 mL

 

3. Sample Preservation

Samples must be stored at -80°C to ensure stability.

 

Note: Please provide details on sample collection and handling.

 

Deliverables

1. Experimental Procedures

2. Relevant Liquid Chromatography and Mass Spectrometry Parameters

3. Detailed Information on Equol

4. Raw Data

5. A Comprehensive Analysis Report

 

At MtoZ Biolabs, we strive to support your research and product development processes by providing accurate and reliable equol analysis results. If you would like to inquire about our services, please feel free to contact us.