Dynamic Light Scattering Analysis Service | Recombinant Collagen
- Sample Type: Recombinant collagen or collagen-like peptides.
- Buffer: Use clear, low-viscosity buffers; avoid surfactants or high salt.
- Storage and Shipping: Store at –80°C. Ship on dry ice with sample details
Understanding the size distribution and aggregation state of recombinant collagen is critical in the development of biologically relevant and stable formulations. Changes in particle size or polydispersity can significantly affect collagen’s solubility, bioavailability, injectability, and interaction with other biomaterials. Dynamic Light Scattering (DLS) is a powerful, non-invasive technique used to assess the hydrodynamic diameter, size distribution, and aggregation behavior of particles in solution. It is widely adopted for protein and nanoparticle analysis, and is particularly well-suited for the characterization of collagen samples.
When light is passed through a colloidal solution of macromolecules like recombinant collagen, fluctuations in light intensity occur due to Brownian motion. These fluctuations are measured and analyzed to determine the diffusion coefficient, which is then used to calculate the hydrodynamic radius (Rh) of the particles. DLS provides a rapid and highly sensitive way to detect changes in molecular size, making it ideal for monitoring batch uniformity, aggregation, formulation effects, and storage stability.
Service at MtoZ Biolabs
MtoZ Biolabs offers comprehensive Dynamic Light Scattering Analysis Services for recombinant collagen, utilizing high-performance systems with advanced temperature control and optimized optical configurations. Our Dynamic Light Scattering Analysis Service supports customizable testing conditions, and provides expert data interpretation to ensure accurate, reproducible, and rapid particle size characterization.
MtoZ Biolabs also provides a full suite of recombinant collagen structural characterization services, including Fourier-Transform Infrared Spectroscopy (FTIR) for secondary structure analysis and Transmission Electron Microscopy (TEM) for direct visualization of fibril morphology. By integrating multiple analytical approaches, we help clients gain a comprehensive understanding of recombinant collagen quality and functionality.
Analysis Workflow
1. Sample Inspection
Assess sample clarity, concentration, and buffer compatibility; perform filtration if necessary.
2. Instrument Calibration
Calibrate the DLS system using certified particle size standards to ensure accuracy.
3. Measurement Setup
Load the sample and set measurement parameters, including temperature and equilibration time.
4. Data Acquisition
Perform multiple scans to collect particle size distribution data with high reproducibility.
5. Data Processing
Analyze results to determine hydrodynamic diameter (Dh), polydispersity index (PDI), and size distribution by intensity.
6. Report Generation
Deliver a comprehensive report including raw data, annotated plots, and expert interpretation.
Service Advantages
✔️Specialized in Collagen Analysis: Extensive experience in analyzing recombinant collagen and collagen-like materials.
✔️High Sensitivity and Non-Invasive: Detects subtle size changes and early-stage aggregation without damaging samples.
✔️Minimal Sample Requirement: Requires only small volumes, suitable for limited or high-value samples.
✔️Flexible Testing Conditions: Supports measurements under different temperatures, buffers, and formulation settings.
✔️Fast Turnaround Time: Efficient workflow enables timely data delivery to support research progress.
Applications
1. Particle Size Characterization: Determine the hydrodynamic diameter and size distribution of recombinant collagen in solution.
2. Aggregation Detection: Identify early-stage aggregation and monitor molecular stability under different conditions.
3. Formulation Development: Evaluate the impact of buffer composition, pH, excipients, or additives on size and dispersion.
4. Batch Consistency Assessment: Compare particle size profiles across different production lots to ensure uniformity.
5. Storage and Shelf-Life Testing: Assess structural stability over time under various storage temperatures and durations.
Sample Submission Suggestions
*Note: Please contact us before shipping for any special requirements or pre-analysis consultation.
Deliverables
1. Comprehensive Experimental Details
2. Raw DLS data files
3. Particle size distribution plots
4. Key parameters such as Hydrodynamic diameter (Dh) and polydispersity index (PDI)
5. Customized report with interpretation
If you are interested in our Dynamic Light Scattering Analysis Service, please feel free to contact us.
Related Services
Recombinant Collagen Structure Characterization Service
Fourier Transform Infrared Spectroscopy Characterization Service | Recombinant Collagen
Recombinant Collagen Transmission Electron Microscopy (TEM) Analysis Service
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