Does Washing Tissue Blocks with Enzyme-Free Water During Western Blot Sample Preparation Affect Subsequent Protein Analysis?
Enzyme-free water is commonly used during tissue or cell washing steps to eliminate potential contaminants such as exogenous proteins, extracellular matrix components, or residual reagents. Its use helps reduce nonspecific background signals and enhances the specificity and reliability of subsequent protein-based assays, including Western blotting.
In most cases, rinsing tissue blocks with enzyme-free water does not significantly interfere with downstream protein analysis. The main purpose of this washing step is to minimize contamination that could compromise signal clarity. However, excessive washing should be avoided, as it may lead to protein loss and negatively affect the recovery or detectability of the target proteins.
MtoZ Biolabs, an integrated chromatography and mass spectrometry (MS) services provider.
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