Deep Phosphoproteomics Service

Phosphorylation is among the most significant types of post-translational modifications of proteins, playing a vital role in regulating protein activity, localization, folding, and interactions. This modification not only influences the relative abundance of proteins but also is crucial in many essential biological processes. Consequently, while studying the "naked" proteome encoded by the genome is important, a thorough exploration of post-translationally modified proteins and their regulatory mechanisms is equally necessary.

 

Nonetheless, conventional phosphoproteomics approaches face several limitations. Techniques based on 2D electrophoresis generally identify only a limited number of phosphorylation sites, making it particularly challenging to detect low-abundance phosphorylated proteins in complex biological samples. Furthermore, traditional 3D analysis methods often lack the resolution required to identify subtle structural changes, resulting in the potential loss of important information.

 

To tackle these challenges, MtoZ Biolabs has implemented the 4D-Label Free technology, which offers Deep Phosphoproteomics Service. This technology integrates dual TIMS (ion mobility separation) and PASEF (parallel accumulation-serial fragmentation) data acquisition modes, significantly improving both the sensitivity and specificity of protein analysis.

 

In 4D proteomics, the abundance of each peptide correlates with its peak area in mass spectrometry (MS1), and the retention time (RT) of identical peptides across different samples remains consistent. This consistency enables quantitative comparisons of relative protein expression levels across various samples. Additionally, the 4D-LFQ technology effectively addresses the limitations of conventional DDA methods concerning low-abundance signal loss, providing more stable qualitative and quantitative data, thus establishing a solid foundation for further research.

 

Analysis Workflow

 

 

Liu, Y. Y. et al. Parasites Vectors. 2024.

 

Service Advantages

1. High Sensitivity: Capable of detecting low-abundance phosphorylated proteins, thereby enhancing the analytical capacity for complex samples.
2. High Throughput: Enables simultaneous analysis of multiple samples, improving experimental efficiency and accommodating large-scale research needs.
3. Strong Accuracy: Integrates multiple quantification techniques to ensure the reliability of results and minimize experimental errors.
4. Comprehensiveness: Suitable for a variety of biological sample types, including cells, tissues, and bodily fluids.

 

Sample Submission Requirements

 

For more sample details, please consult our technical team.

 

Our Deep Phosphoproteomics Service provides high-quality data support, advancing your scientific research and applications. For any inquiries or requirements, please contact us.