Data Independent Acquisition Mass Spectrometry Service

Data Independent Acquisition Mass Spectrometry is transforming the analysis of complex biological samples with its innovative approach. By capturing the mass and intensity of all ions in each scan, Data Independent Acquisition Mass Spectrometry  enables comprehensive and accurate analysis of peptides and proteins. This technology excels in analyzing complex samples, improving reproducibility, and delivering precise quantitative results, making it indispensable for cancer research, clinical biomarker discovery, and drug screening.

 

At MtoZ Biolabs, our Data Independent Acquisition Mass Spectrometry Service is designed to meet the needs of researchers and enterprises by offering high-quality data and tailored solutions. With optimized workflows and advanced instruments, we ensure efficient data acquisition and analysis to drive breakthroughs in biomedical research and drug development.

 

Technical Principles

The core principle of Data Independent Acquisition Mass Spectrometry (DIA-MS) lies in its unique data acquisition strategy. Traditional tandem mass spectrometry, known as Data Dependent Acquisition (DDA), selectively analyzes only specific ions from each scan, often missing low-abundance ions and compromising data completeness. In contrast, Data Independent Acquisition Mass Spectrometry uses predefined mass range windows to capture signals from all ions in every scan, significantly improving data comprehensiveness and accuracy.

 

During Data Independent Acquisition Mass Spectrometry analysis, the mass spectrometer acquires ion signals across segmented mass ranges simultaneously. Advanced deconvolution algorithms then process these signals, reconstructing peptide mass spectra with high precision. This approach enables accurate protein quantification, enhances the detection of low-abundance proteins, and supports the analysis of complex protein interactions, providing a robust foundation for proteomic studies.

 



 

Figure 1. Comparison of DDA-MS and DIA-MS

Krasny, L. et al. Mol. Omics. 2021.

 

 

Technical Advantages

• High Throughput: Full-spectrum data acquisition streamlines large-scale analyses.
• Enhanced Sensitivity: Captures low-abundance proteins with unmatched precision.
• Robust Reproducibility: Independent acquisition minimizes errors, ensuring reliable results.
• Adaptable for Complexity: Handles diverse biological samples, from serum to tissue extracts.
• Scalable Solutions: Meets the needs of both small-scale and large-scale studies.

 

Analysis Workflow

At MtoZ Biolabs, our Data Independent Acquisition Mass Spectrometry Service delivers comprehensive proteomics solutions through a well-structured workflow that guarantees precision and reliability at every stage.

1. Sample Preparation

Samples undergo standardized protein extraction and enzymatic digestion procedures, with rigorous quality control measures to ensure data accuracy and reproducibility.

 

2. Mass Spectrometry Analysis

Utilizing Data Independent Acquisition Mass Spectrometry technology, mass spectrometers capture all ion signals across predefined mass windows during each scan, enabling extensive data acquisition with high sensitivity and precision.

 

3. Data Deconvolution and Quantitative Analysis

Specialized algorithms process DIA data to deconvolute ion signals, reconstructing peptide mass spectra accurately. This enables reliable qualitative and quantitative analysis of proteins across various sample types.

 

4. Data Analysis and Reporting

Comprehensive reports are generated, detailing protein composition, abundance changes, and other key parameters. These reports offer actionable insights for understanding biological mechanisms and guiding decision-making in research and development.

 



Krasny, L. et al. Mol. Omics. 2021.

 

Sample Submission Suggestions

1. Sample Types

Serum, plasma, urine, tissue extracts, and other biological samples.

 

2. Sample Volume

At least 200 μL of liquid sample or 200 mg of solid sample.

 

3. Sample Preservation

Store samples at -20°C or lower to prevent degradation, avoiding repeated freeze-thaw cycles.

 

Note: Provide details on sample collection and handling.

 

For more specific sample submission requirements, please consult our technical team.

 

Why Choose MtoZ Biolabs?

1. Advanced Analysis Platform: MtoZ Biolabs established an advanced Data Independent Acquisition Mass Spectrometry platform, guaranteeing reliable, fast, and highly accurate analysis service.

2. One-Time-Charge: Our pricing is transparent, no hidden fees or additional costs.

3. High-Data-Quality: Deep data coverage with strict data quality control. AI-powered bioinformatics platform integrate all proteomics analysis data, providing clients with a comprehensive data report.

4. Customized Service: Tailored to the specific research needs of our clients, we offer flexible experimental design and personalized data analysis to ensure the achievement of research goals to the fullest extent.

 

Case Study

Case 1: Data Independent Acquisition Mass Spectrometry Facilitates Crustacean Neuropeptide Spectral Library Construction

In this study, Data Independent Acquisition Mass Spectrometry was employed to systematically identify and characterize crustacean neuropeptides, resulting in the construction of a high-quality spectral library. This library provides a reliable reference for identifying and quantifying complex peptides, significantly improving efficiency and accuracy in neuropeptide research.

 



Fields, L. et al. Proteomics. 2024.

 

Case 2: Data Independent Acquisition Mass Spectrometry Reveals Gravity-Responsive Proteins in Mouse Serum During Spaceflight

By applying Data Independent Acquisition Mass Spectrometry, researchers quantitatively analyzed serum proteins from space-flown mice and identified proteins with differential expression linked to gravitational changes. This analysis captured low-abundance protein expression variations with high precision, providing critical data for understanding the molecular impacts of microgravity on biological systems.

 



 Kimura, Y. et al. Proteomics. 2024.

 

What Could be Included in the Report?

1. Comprehensive Experimental Details

2. Materials, Instruments, and Methods

3. Total Ion Chromatogram & Quality Control Assessment

4. Data Analysis, Preprocessing, and Estimation

5. Bioinformatics Analysis

6. Raw Data Files

 

MtoZ Biolabs offers cutting-edge Data Independent Acquisition Mass Spectrometry Service tailored to your research needs. Contact us to learn how we can empower your projects with precise, reliable, and efficient proteomics solutions.