D-ribulose-1,5-bisphosphate Analysis Service

    D-ribulose-1,5-bisphosphate (RuBP) is a critical organic compound involved in the photosynthetic carbon fixation process, specifically in the Calvin cycle. Structurally, RuBP is a pentose sugar phosphate with the molecular formula C₅H₁₂O₁₁P₂, featuring two phosphate groups attached to the first and fifth carbon atoms of a ribulose sugar. As the primary CO₂ acceptor molecule in the Calvin cycle, RuBP plays a pivotal role in the conversion of inorganic carbon into organic molecules, thereby serving as the foundation for the biosynthesis of glucose and other carbohydrates in plants.

     

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    Figure 1. The Structure of D-ribulose-1,5-bisphosphate

     

    RuBP is synthesized in the chloroplasts of plant cells and is continually regenerated through a series of enzyme-mediated reactions. Its significance extends beyond its primary role in photosynthesis; RuBP is also involved in regulating the activity of ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBisCO), the enzyme responsible for catalyzing the first step in the carbon fixation pathway. Due to its central role in photosynthetic metabolism, the concentration and dynamics of RuBP within the chloroplast are crucial for maintaining optimal photosynthetic efficiency and overall plant health.

     

    At MtoZ Biolabs, we understand the importance of accurate, precise, and sensitive analysis of D-ribulose-1,5-bisphosphate. Our state-of-the-art mass spectrometry facilities and specialized expertise in metabolomics allow us to provide comprehensive, reliable, and detailed analysis of this compound, unveiling crucial insights into your research.

     

    We provide the following:

    • Analysis of D-ribulose-1,5-bisphosphate concentration
    • Detailed report on metabolic pathways involving RuBP
    • Interpretation and insights into the data

     

    Analysis Workflow 

     

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    Service Advantages

    • Continuously optimized experimental protocols and advanced instrument
    • Professional experimental design
    • Rapid turnaround time
    • High accuracy, specificity, and sensitivity

     

    Applications

    Our D-ribulose-1,5-bisphosphate Analysis Service has a wide range of applications, including:

    • Studying photosynthesis mechanisms
    • Investigating factors influencing plant growth and crop yield
    • Developing strategies for bioenergy production
    • Conducting environmental impact studies

     

    Sample Submission Requirements

    1. Sample Types

    • Plant tissues and other biological samples.
    • More than 3 materials with the same condition should be selected for each sample. 

     

    2. Sample Volume

    Plant tissue ≥ 100 mg

     

    3. Sample Preservation

    Samples should be stored at -80°C to maintain stability.

     

    Detailed instructions on sample collection and handling will be provided.

     

    Deliverables

    1. Experimental Procedures

    2. Relevant Liquid Chromatography and Mass Spectrometry Parameters

    3. Detailed Information on D-ribulose-1,5-bisphosphate

    4. Raw Data

    5. Custom Analysis Report

     

    Contact us today to learn more about how our D-ribulose-1,5-bisphosphate Analysis Service can support your research.

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