Cytosine Analysis Service
- Support in the discovery of new disease biomarkers
- Enhancement of disease diagnosis
- Accelerating the discovery of innovative therapeutics
- Unveiling new insights into disease mechanisms
- Animal tissue ≥ 100 mg
- Plant tissue ≥ 200 mg
- Serum/ Plasma ≥ 200 μL
- Urine ≥ 2 mL
Cytosine is a nitrogen-containing organic compound that belongs to the class of pyrimidine bases, with the chemical formula C₄H₅N₃O. Its structure consists of a pyrimidine ring, an amino group (-NH₂), and a carbonyl group (=O). In DNA and RNA, cytosine pairs with deoxyadenine (dA) and adenine (A) to form base pairs. Cytosine can be synthesized in living organisms through nucleotide metabolic pathways, primarily derived from the synthesis of nucleotides. It is present in various organisms, including bacteria, plants, and animals. As a component of DNA and RNA, cytosine plays a crucial role in the storage and transmission of genetic information. It is important in protein synthesis, gene expression regulation, and cellular metabolism. Additionally, cytosine is essential for cell division and growth.
The metabolism of cytosine primarily occurs through deamination and reduction reactions, resulting in the formation of uracil and deoxycytidine, as well as cytidine monophosphate (CMP). These metabolites play significant roles in RNA and DNA, participating in transcription and translation processes, affecting gene expression, ensuring the accurate transmission of genetic information, and contributing to cellular metabolism, especially in energy metabolism and nucleic acid synthesis. The content of cytosine is closely related to plant growth and development. Analyzing its levels can help reveal the physiological responses of plants under stress conditions, such as drought, salinity, and disease. By studying cytosine and its metabolites, researchers can elucidate nucleotide synthesis and its roles in cellular metabolism, providing a foundation for understanding cellular functions and metabolic regulation.
Figure 1. The Structure of Cytosine
Mass spectrometry-based metabolomics methods can simultaneously detect and quantify thousands of metabolic features and are widely used for the metabolic analysis of various compounds. MtoZ Biolabs utilizes an LC-MS-based metabolomics research platform for the analysis of cytosine. We ensure the accuracy of the quantification of cytosine and its metabolites, as well as the comprehensiveness and precision of metabolite identification. If you would like to learn more about our cytosine analysis services, please feel free to contact us, and we will be more than happy to assist you.
Analysis Workflow
Service Advantages
1. Experienced: MtoZ Biolabs has a professional research team and advanced technical equipment, capable of providing high-quality services and support to meet the specific needs of clients.
2. Wide Applicability: It is suitable for various types of samples, including plants, animals, and biological fluids, making it applicable across a broad range of fields.
3. High Throughput: Mass spectrometry technology can analyze multiple metabolites simultaneously in a short time, making it suitable for high-throughput analysis of large-scale samples.
4. Targeted Compounds: Targeted metabolomics focuses on specific metabolites, effectively eliminating interference signals and enhancing the reliability of the data.
5. Strong Detection Capability: Mass spectrometry technology can detect low concentrations of metabolites, making it suitable for analyzing rare or complex components in biological samples.
Applications
Sample Submission Requirements
1. Sample Types
Serum, plasma, urine, cells, tissues, and other biological samples. A minimum of 3 samples under the same condition is required for each analysis.
2. Sample Volume
3. Sample Preservation
Samples should be stored at -80°C to ensure stability.
Note: Please provide detailed information on sample collection and handling.
Deliverables
1. Experimental Procedures
2. Relevant Liquid Chromatography and Mass Spectrometry Parameters
3. Detailed Information on Cytosine
4. Raw Data
5. Custom Analysis Report
Our Cytosine Analysis Service is designed to assist your research and product development endeavors by providing accurate and reproducible results.
How to order?
