Conservation Analysis of Amino Acid Sequences: Key Insights into Protein Function

    The aim of sequence conservation analysis is to determine the degree of similarity between protein or other amino acid sequences across different species or family members. This analysis can reveal which amino acid residues are crucial in terms of function or structure, as these residues have been preserved throughout evolution.

     

    The general steps to perform amino acid sequence conservation analysis include:

     

    Collection of Sequence Data

    Protein sequences of different species or different members of the same species are collected from public databases such as NCBI, Uniprot, etc.

     

    Sequence Alignment

    These sequences are aligned using software tools such as BLAST, Clustal Omega, MAFFT, etc., to perform multiple sequence alignment (MSA) and identify similarities and differences.

     

    Identifying Conserved Regions

    In the alignment results, highly similar or identical sequences of amino acids usually indicate these regions are important in terms of structure or function. These regions are often considered "conserved" as they are preserved across different sequences, even if these sequences come from different species.

     

    Analysis and Interpretation

    Based on the position and nature of the conserved regions, as well as their correspondence with known functional or structural domains, the biological significance of these conservations is analyzed. For example, a highly conserved region might be an active site, a binding site, or a particularly important area for maintaining the three-dimensional structure of the protein.

     

    Further Experimental Validation

    Based on the predictions from the conservation analysis, experiments can be designed (such as site-directed mutagenesis, functional identification, etc.) to validate the exact roles of certain amino acid residues.

    MtoZ Biolabs, an integrated chromatography and mass spectrometry (MS) services provider.

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