Can the Protein Precipitate Be Removed by Vacuum Filtration in the Sevag Deproteinization Method?

The Sevag method is commonly employed to remove proteins from protein-containing liquid samples. This approach utilizes a mixed solvent of chloroform and isopropanol to denature and separate proteins, while nucleic acids such as DNA or RNA remain in the aqueous phase. During the process, proteins in the sample are denatured and aggregated by the organic solvents, forming a precipitate. This precipitate can subsequently be removed from the sample by vacuum filtration or centrifugation, thereby effectively eliminating proteins from the preparation.

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