Can Saccharomyces cerevisiae S288C Be Used as the Reference Genome for Transcriptome Sequencing of the BY4741 Strain?
BY4741 and S288C are both commonly used laboratory strains of Saccharomyces cerevisiae. S288C is the reference strain of yeast, with its genome being the first to be fully sequenced, and it is widely used as a reference in various genomic tools and databases. BY4741, on the other hand, is a haploid strain derived from S288C and is frequently employed in genomic studies, including gene knockout experiments.
If you are conducting transcriptome sequencing (RNA-Seq) and require a reference genome for alignment, S288C can indeed serve as a reference for BY4741. In fact, a substantial number of studies involving BY4741 have utilized the S288C genome as a reference, as observed in the literature.
However, it is important to note that although BY4741 and S288C are highly similar at the genomic level, slight differences may exist due to specific gene knockouts or mutations. In most applications, these minor discrepancies are unlikely to affect the results significantly. Nonetheless, if your research focuses on particular genes or genomic regions, it is advisable to verify that no critical differences exist between your experimental strain and the reference genome.
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