Can Protein Samples in Gels Be Stored Long-Term, Shipped Safely, and Used for Mass Spectrometry
Protein samples embedded in gels can be stored long-term under appropriate conditions; however, the following considerations are important:
1. Storage Conditions
Gel slices should be stored at 4°C or lower to minimize protein degradation. They should be tightly sealed with laboratory-grade plastic film, and moistened paper towels should be placed around the gel to prevent drying. Additionally, soaking the gel slices in a buffer containing 50% glycerol may further help retain moisture and prevent desiccation.
2. Transportation
During transportation, it is essential to maintain both hydration and a cold environment for the gel slices. Place them in a sealed plastic bag or container along with moistened paper towels to retain humidity. Use ice packs or dry ice to preserve a low temperature throughout transit, and ensure the samples are protected from mechanical shocks or excessive vibrations.
3. Impact on Mass Spectrometry Identification
Extended storage and transport can potentially affect the quality of protein samples. Proteins may undergo partial degradation, particularly under suboptimal storage conditions. Furthermore, chemical modifications such as oxidation may occur. These factors can introduce variability in mass spectrometry results. Nonetheless, with proper storage and handling, such effects are typically minimized.
It is important to emphasize that, although protein samples in gels can be preserved for extended periods, mass spectrometry analysis should ideally be performed as soon as possible after protein preparation to ensure the most accurate results.
MtoZ Biolabs, an integrated chromatography and mass spectrometry (MS) services provider.
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