Can I Reboil My WB Sample After Storing It in the Fridge
Yes. If the sample has undergone prior boiling and has been stored at 4°C, it is recommended to reheat it before use to ensure that the components in the loading buffer, such as SDS and DTT (or β-mercaptoethanol), function effectively, allowing complete protein denaturation. Before reheating, ensure that the sample is fully thawed and gently mixed to prevent phase separation or precipitation of its components, which could affect experimental results.
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