Can Guanidine Hydrochloride Be Used in Mass Spectrometry
No, guanidine hydrochloride (Gu-HCl) is commonly employed as a protein denaturant to unfold the tertiary structure of proteins prior to mass spectrometry (MS) analysis in proteomics. Its application can enhance peptide extraction efficiency and increase the coverage of peptide identification. However, guanidine hydrochloride itself may interfere with MS analysis by causing signal suppression and elevating background noise.
In practice, protein samples treated with guanidine hydrochloride typically require appropriate purification or dilution steps to mitigate its interference in MS analysis. Excess guanidine hydrochloride can be removed via dialysis, dilution, or solid-phase extraction methods (e.g., C18 columns). Such pretreatment renders the sample more suitable for subsequent protein mass spectrometry analysis.
MtoZ Biolabs, an integrated chromatography and mass spectrometry (MS) services provider.
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