Biomarker Discovery Service
Biomarker Discovery Service enables the identification of phosphorylation-based biomarkers, which are essential for early disease detection, prognosis, and therapeutic monitoring. These biomarkers provide measurable indicators of cellular state, disease progression, and drug response. Phosphorylation-based biomarkers play a crucial role in signaling regulation and disease pathology, offering insights into kinase activity, pathway dysregulation, and molecular mechanisms in cancer, neurodegenerative disorders, metabolic diseases, and immune dysfunction. However, identifying clinically relevant phosphorylation biomarkers remains challenging due to their low abundance, transient nature, and complex regulatory mechanisms.
MtoZ Biolabs offers a comprehensive Biomarker Discovery Service designed to systematically identify and validate biomarkers for clinical and translational research. Our Biomarker Discovery Service integrates deep phosphoproteome profiling, targeted validation, and pathway analysis to uncover disease-specific phosphorylation patterns, providing quantitative insights into disease mechanisms and therapeutic targets.
Analysis Workflow
1. Protein Extraction: Extract proteins from biofluids, tissues, or cell lysates under stringent conditions.
2. Protein Digestion: Perform tryptic digestion to generate peptides while preserving phosphorylation sites.
3. Phosphopeptide Enrichment: Utilize IMAC or TiO₂-based affinity purification to selectively capture phosphopeptides.
4. LC-MS/MS Analysis – Conduct high-resolution DIA-MS for comprehensive phosphoproteome profiling.
5. Data Processing and Normalization: Apply advanced computational pipelines for peak extraction, quantification, and batch correction.
6. Bioinformatics Analysis: Perform differential phosphorylation analysis, kinase-substrate network mapping, and functional annotation to identify key phosphorylation biomarkers and their regulatory pathways.
Service Advantages
✅State-of-the-art Biomarker Discovery Service platforms allow us to deliver accurate and reliable results.
✅A team of experts in mass spectrometry and proteomics provides professional data analysis and interpretation.
✅One-stop service from sample preparation, LC-MS/MS analysis to data analysis, saving your time and efforts.
✅Competitive price with high-quality service.
Applications
1. Early Disease Detection
Identifies phosphorylation-based biomarkers for early diagnosis of cancer, neurodegenerative diseases, and metabolic disorders, improving clinical intervention strategies.
2. Therapeutic Target Identification
Screens for phosphorylation-regulated proteins involved in disease progression, facilitating the discovery of novel drug targets and kinase inhibitors.
3. Prognostic and Predictive Biomarkers
Links phosphorylation signatures to patient outcomes, aiding in risk stratification and personalized medicine.
Case Study
Colorectal cancer (CRC) is highly heterogeneous, and despite extensive genomic studies, clinically actionable biomarkers remain limited. A proteogenomic study systematically analyzed paired tumor and normal tissues, integrating phosphoproteomics and genomics to identify cancer-associated proteins, phosphosites, and therapeutic targets. Phosphoproteomics linked Rb phosphorylation to increased proliferation and reduced apoptosis, suggesting a rationale for targeting Rb phosphorylation in CRC treatment. Additionally, MSI-H tumors exhibited increased glycolysis and reduced CD8+ T cell infiltration, highlighting glycolysis as a potential target to enhance immune checkpoint therapy. The study also identified novel cancer-specific phosphosites, advancing biomarker discovery and precision oncology. This case underscores the value of Biomarker Discovery Service, which utilizes high-resolution phosphoproteomics and bioinformatics to systematically uncover disease-relevant phosphorylation events, providing insights into tumor progression, drug resistance, and therapeutic development.
Figure 1. Rb Phosphorylation as a Driver and Therapeutic Target in Colon Cancer
Deliverables
1. Comprehensive Experimental Details
2. Materials, Instruments, and Methods
3. Relevant Liquid Chromatography and Mass Spectrometry Parameters
4. The Detailed Information of Biomarker Discovery
5. Mass Spectrometry Image
6. Raw Data
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