Analysis of 3' Poly(A) Tail in Human Cell mRNA

Messenger RNA (mRNA) in human cells is a key intermediate product of gene expression, carrying genetic information transcribed from DNA to protein translation. The maturation and stability of mRNA are regulated by a series of post-transcriptional modifications, the most important of which is the addition of a poly(A) tail at the 3' end. This involves the addition of a string of Polyadenylate sequences (abbreviated as poly(A)) at the 3' end of mRNA. The poly(A) tail is crucial for mRNA nuclear export, stability, translation efficiency, and eventual degradation. For example, a longer poly(A) tail is generally associated with higher translation efficiency and mRNA stability, while shortening of the poly(A) tail is an important signal for mRNA degradation.

 

MtoZ Biolabs uses advanced technology to deeply analyze the 3' Poly(A) tailing of mRNA in human cells, providing valuable molecular-level information for understanding cellular functions, disease mechanisms, and new drug development.

 

Service Advantages

1. High-Precision Measurement

Using quantitative real-time PCR (qRT-PCR) technology to accurately measure the length and number of Poly(A) tails of specific mRNA molecules, ensuring high accuracy of the results.

2. Comprehensive Sequence Analysis

Combined with high-throughput sequencing technology, it can analyze mRNA's 3' Poly(A) tailing pattern at the whole-genome level, revealing its differences in different cell types and states.

3. Advanced Bioinformatics Applications

Using advanced bioinformatics analysis methods to deeply explore the impact of Poly(A) tailing on post-transcriptional regulation of mRNA, providing strong data support for understanding its function.

4. RNA Immunoprecipitation Sequencing (RIP-seq)

With RIP-seq technology, it can identify mRNAs associated with specific Poly(A) binding proteins, further exploring the complex network of mRNA tailing and protein interactions.

 

Applications

1. Disease Mechanism Research

Analyzing changes in mRNA tailing in human diseases such as cancer and neurodegenerative diseases, providing key insights for revealing pathological mechanisms.

2. Gene Expression Regulation Research

Deeply understanding the role of mRNA tailing in regulating gene expression, providing basic data for molecular biology research.

3. Drug Action Mechanism Research

By analyzing how drugs affect the 3' Poly(A) tailing of mRNA, the molecular mechanism of drugs is revealed.

4. Post-Transcriptional Regulation Research

Studying the dynamic changes of mRNA tailing in different cell states, providing a new perspective for understanding the molecular mechanism of cells' response to environmental changes.

 

Through human cell mRNA 3' Poly(A) tailing analysis technology, researchers can explore the complex role of mRNA in gene expression regulation more deeply, providing new directions for biomedical research. We are committed to providing customers with the most advanced analysis services, supporting global scientists in their exploration and discovery in the field of life sciences.