Amino Acid HPLC Analysis Service

Amino acids are the fundamental building blocks of proteins and the basis of life metabolism. They are widely present in food, pharmaceuticals, and life sciences, playing a significant role in scientific research and product development. Amino acid HPLC analysis service employs various chromatographic columns and mobile phases to separate amino acids based on their chemical and physical properties. Most amino acids lack UV absorption and fluorescence emission properties, and standard refractive index detectors are not sensitive enough for their detection. To improve sensitivity and separation selectivity, amino acids are typically derivatized before high-performance liquid chromatography (HPLC) analysis.

 

Services at MtoZ Biolabs

Leveraging amino acid derivatization technology and HPLC analysis, MtoZ Biolabs provides a one-stop amino acid HPLC analysis service to efficiently and accurately analyze the amino acid composition of protein (peptide) drugs. By using calibration with reference standards, we ensure precision and accuracy in testing. Each analysis undergoes stringent quality control to deliver high-precision and high-accuracy results.

 

The amino acid HPLC analysis service provided by MtoZ Biolabs primarily involves two critical steps: (1) Protein and peptide samples are hydrolyzed into individual amino acids. (2) Pre-Column Derivatization, MtoZ Biolabs utilizes phenyl isothiocyanate (PITC) or 6-aminoquinolyl-N-hydroxysuccinimidyl carbamate (AQC) for amino acid derivatization. The resulting derivatives are subjected to gradient elution using HPLC. This process separates different amino acid derivatives, and their concentrations are quantified using external standards. The analysis provides detailed information on the amino acid composition and content within protein samples.

 



Figure 1. Analysis Process of Amino Acid Composition of Peptide/Protein Samples.

 



Bosch, L. et al. J. Chromatogr. B, 2005

Figure 2. Chromatographic Analysis of Amino Acids in Infant Food.

 

Service Advantages

1. Advanced Analytical Platform

MtoZ Biolabs has established a comprehensive HPLC-based amino acid composition analysis platform for antibody protein drugs, peptide drugs, vaccine drugs, PEGylated drugs, and other recombinant protein drugs. This platform ensures reliable, fast, and highly precise analytical services.

 

2. Transparent Pricing

Our pricing structure is transparent, with no hidden fees or additional fees.

 

3. High-Quality Data

We employ strict quality control measures to deliver comprehensive and accurate data reports.

 

4. Customized Research Solutions

MtoZ Biolabs offers tailored services to address your unique research questions and experimental requirements.

 

Sample Submission Suggestions

1. Sample Types 

We accommodate a wide range of sample types, including amino acids, peptides, polypeptides, proteins, and antibodies.

 

2. Salt Concentration

Samples with excessively high salt concentrations should be avoided.

 

3. Buffer Solutions

Avoid buffers containing large amounts of primary amines, such as TRIS or glycine, as these can interfere with ninhydrin reactions and result in inaccurate quantification of specific amino acids.

 

4. Impurities

Ensure that samples are free from impurities such as fingerprints or dust, as these contaminants may contain human proteins that can interfere with detection results.

 

5. Sample Amount

The recommended sample amount is >2 mg. Please provide sufficient solid material to allow accurate weighing during the analysis. You may choose one or three replicates for amino acid determination. For highly accurate protein concentration measurements, we recommend triplicate biological replicates.

Note: For special requirements or assistance with sample preparation, please contact us.

 

Applications

1. Food Industry

Amino acid HPLC analysis service can be used to detect amino acid components in food, evaluating nutritional value and quality.

 

2. Biopharmaceutical Industry

Amino acid composition analysis by HPLC is used to quantify amino acid content in drugs, perform quality control, evaluate protein quality and purity, monitor metabolic changes, and study protein composition and structure.

 

3. Other Fields

Amino acid HPLC analysis service is also applicable to the analysis of plant sterols, plant pigments, phytohormones, alkaloids, and other components.

 

Case Study

1. Simultaneous Analysis of 26 Physiological Amino Acids and Total Cysteine/Homocysteine in Plasma Using HPLC 

The quantification of plasma amino acid levels has significant clinical implications for disease diagnosis, therapeutic monitoring, nutritional assessment, and physiological research. The study employed HPLC with 6-aminoquinolyl-N-hydroxysuccinimidyl carbamate (AQC) as a pre-column derivatization reagent to simultaneously analyze 26 physiological amino acids in plasma. Amino acid HPLC analysis service was applied in metabolic laboratories to establish reference ranges for plasma amino acids and identify metabolic disorders such as classical homocystinuria, cobalamin deficiency, nonketotic hyperglycinemia, hyperprolinemia, ketotic hyperglycinemia, urea cycle defects, and maple syrup urine disease. HPLC not only provided quantitative data on amino acids in plasma but also expanded its application to identify low-level amino acid metabolites, demonstrating significant potential for clinical research.

 



Gaurav, S.et al. Amino Acids. 2014.

Figure 3. Chromatograms of Amino Acids in Plasma of Control Group and Representatives of Different Metabolic Diseases (Hyperhomocysteinemia, Nonketotic Hyperglycemia, Hyperprolinemia).

 

Deliverables

1. Comprehensive Experimental Details

 

2. Materials, Instruments, and Methods

 

3. Total Ion Chromatogram & Quality Control Assessment 

 

4. Data Analysis, Preprocessing, and Estimation

 

5. Raw Data Files

 

MtoZ Biolabs, an integrated Chromatography and Mass Spectrometry (MS) Services Provider, provides advanced proteomics, metabolomics, and biopharmaceutical analysis services to researchers in biochemistry, biotechnology, and biopharmaceutical fields. Our ultimate aim is to provide more rapid, high-throughput, and cost-effective analysis, with exceptional data quality and minimal sample consumption. Free project evaluation, welcome to learn more details!