Alpha-Ketoglutaric Acid Analysis Service
- Continuously optimized experimental protocols and advanced instrument
- Professional experimental design
- Rapid turnaround time
- High accuracy, specificity, and sensitivit
- Identification and quantification of alpha-ketoglutaric acid in biological samples
- Evaluation of metabolic disorders related to alpha-ketoglutaric acid
- Drug efficacy and toxicity studies
Alpha-ketoglutaric acid (α-KG) belongs to the family of keto acids, characterized by a ketone group attached to the second carbon atom of the glutaric acid backbone. It serves as a key intermediate in various metabolic pathways, particularly in the citric acid cycle (Krebs cycle), where it plays a crucial role in energy production. α-KG is also involved in amino acid metabolism, acting as a precursor for the synthesis of glutamate and other amino acids. In mammals, α-ketoglutaric acid is vital for the proper functioning of the citric acid cycle, where it facilitates the conversion of isocitrate to succinyl-CoA, a critical step in cellular respiration. This process is essential for generating ATP, the primary energy currency of the cell. Beyond energy metabolism, α-KG is involved in several biological processes, including the regulation of nitrogen balance, the detoxification of ammonia, and the synthesis of neurotransmitters like glutamate.
The levels and metabolism of α-ketoglutaric acid are linked to various physiological and pathological conditions. For instance, abnormal α-KG levels have been associated with metabolic disorders, cancer, and neurodegenerative diseases. Therefore, the precise quantification and structural characterization of α-KG in biological samples are crucial for understanding its role in disease progression and elucidating its mechanism of action.
Figure 1. The Structure of Alpha-Ketoglutaric Acid
MtoZ Biolabs offers a comprehensive Alpha-Ketoglutaric Acid Analysis Service, backed by our state-of-the-art liquid chromatography-mass spectrometry (LC-MS) platform and experienced metabolomics team. Our service guarantees high sensitivity and precision in the analysis of alpha-ketoglutaric acid.
Analysis Workflow
Service Advantages
Applications
Sample Submission Requirements
1. Sample Types
Serum, plasma, urine, tissues and other biological samples. Each sample should be accompanied by at least 3 replicates under the same condition.
2. Sample Volume
A minimum of 200 μL for liquid samples; A minimum of 200 mg for solid tissues.
3. Sample Preservation
Samples should be stored at -80°C to ensure stability.
Note: Please include detailed information about sample collection and handling.
Deliverables
1. Experimental Procedures
2. Relevant Liquid Chromatography and Mass Spectrometry Parameters
3. Detailed Information on Alpha-Ketoglutaric Acid
4. Raw Data
5. Custom Analysis Report
At MtoZ Biolabs, our alpha-ketoglutaric acid analysis service aims to expedite your research and product development endeavors by providing accurate, reliable, and detailed results.
How to order?
