Absolute Quantitative Lipidomics Analysis Service

    Absolute quantitative lipidomics analysis leverages high-throughput mass spectrometry to systematically identify and accurately quantify lipid molecules in biological samples. As essential components of cell membranes, lipids play crucial roles in processes such as signal transduction, energy metabolism, and disease regulation. Through lipidomics research, scientists can measure the precise concentrations of lipids and investigate their dynamic changes and functional roles under various physiological or pathological conditions. Unlike traditional relative quantification methods, absolute quantification uses isotope-labeled internal standards to provide highly accurate concentration data for each lipid, significantly improving data reliability and sensitivity.

     

    MtoZ Biolabs offers a comprehensive Absolute Quantitative Lipidomics Analysis Service using the UPLC-Orbitrap mass spectrometry platform, paired with LipidSearch software and a range of isotope-labeled internal standards. This service enables precise absolute quantification of lipid molecules, supporting research in fields such as metabolic regulation, disease biomarker discovery, and clinical studies.

     

    Analysis Workflow

     

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    Data Analysis

     

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    Service Advantages

    1. High-Resolution Mass Spectrometry

    Provides precise identification of lipid molecules, with the ability to distinguish structurally similar lipids, enhancing the accuracy of lipid identification.

     

    2. Internal Standard Correction

    By utilizing isotope-labeled internal standards, batch effects are minimized, ensuring reliable and accurate lipid quantification.

     

    3. In-Depth Lipid Feature Analysis

    Enables detailed analysis of lipid content, chain length, and saturation levels, facilitating more advanced data exploration and interpretation.

     

    4. Comprehensive Lipidomic Coverage

    LipidSearch supports the qualitative and quantitative analysis of 8 major lipid classes, 300 sub-classes, and over 1.7 million lipid ions, including phospholipids, glycerides, cholesterol esters, and sphingolipids.

     

    Sample Submission Requirements

     

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    For more sample details, please consult our technical team.

     

    Applications

    • Lipid biomarker screening research
    • Exosome composition and function research
    • Glycolipid metabolism research
    • Membrane/subcellular organelle composition and function research
    • Food nutrition and quality research

     

    Deliverables

    What Could be Included in the Report?

    1. Comprehensive Experimental details

    2. Materials, Instruments, and Methods

    3. Total Ion Chromatogram & Quality Control Assessment (project-dependent)

    4. Data Analysis, Preprocessing, and Estimation (project-dependent)

    5. Bioinformatics Analysis

    6. Raw Data Files

     

    MtoZ Biolabs offers a state-of-the-art Absolute Quantitative Lipidomics Analysis service, providing researchers with high-quality lipid data and comprehensive insights into lipid functions and mechanisms across various biological processes. Free project evaluation, welcome to learn more details!

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