7-methylguanosine Analysis Service

7-methylguanosine (m7G) is a modified nucleoside that arises from methylation of the amino group at position 7 of guanosine. It has been widely observed in various biological substances, particularly in eukaryotic mRNA, where it is known as a cap structure at the 5' end. This cap structure plays a significant role in mRNA transcription, splicing, nuclear export, and translation. Additionally, m7G is involved in numerous biological processes and diseases, such as oxidative stress, inflammation, and cancer. The presence, absence, and quantity of m7G can provide critical insights into these processes and conditions, making it a significant target for numerous research studies. Investigating the metabolism and function of m7G can shed light on the mechanisms of related diseases and provide potential therapeutic targets.

 



 

Figure 1. The Structure of 7-methylguanosine

 

At MtoZ Biolabs, we offer comprehensive 7-methylguanosine analysis service, designed to meet the diverse needs of researchers in the field. Our service includes:

• Quantification of m7G levels in biological samples using advanced LC-MS/MS platform.
• Characterization of RNA modifications to understand their biological roles and implications.
• Analysis of mRNA stability and translation efficiency to explore the functional impact of m7G.
• Comparative studies of m7G levels in various conditions, such as stress response and disease states.

 

By utilizing our expertise in mass spectrometry and metabolomics, MtoZ Biolabs aims to support your research endeavors, paving the way for new discoveries and therapeutic applications.

 

Analysis Workflow

 



 

Service Advantages

• State-of-the-art LC-MS/MS platforms allow us to accurately and reliably quantify 7-methylguanosine in various types of samples.
• A team of experts in mass spectrometry and metabolomics provides professional data analysis and interpretation.
• One-stop service from sample preparation, LC-MS/MS analysis to data analysis, saving your time and efforts.
• Competitive price with high-quality service.

 

Applications

Our m7G analysis service can be applied to various research fields, including but not limited to:

• Biomarker Discovery
• Drug Discovery and Development
• Pathological Research

 

Sample Submission Requirements

1. Sample Types

Serum, plasma, urine, tissues, and other biological samples. It is recommended to prepare at least 3 biological replicates for each sample type.

 

2. Sample Volume

• Animal tissue ≥ 100 mg
• Plant tissue ≥ 200 mg
• Serum/ Plasma ≥ 200 μL
• Urine ≥ 2 mL

 

3. Storage and Shipping

Samples should be stored at -80℃ and shipped with dry ice. Avoid repeated cycles of freezing and thawing

 

Note: Please provide details on sample collection and handling.

 

Deliverables

1. Experimental Procedures

2. Relevant Liquid Chromatography and Mass Spectrometry Parameters

3. Detailed Information on 7-methylguanosine

4. Raw Data

5. Custom Analysis Report

 

For more information or to discuss your project needs, please contact us. Together, we can solve the puzzles of your research.