7α,27-Dihydroxycholesterol Analysis Service | Steroid hormones

7α,27-Dihydroxycholesterol (7α,27-OHC) is a dihydroxylated derivative of cholesterol, characterized by the presence of two hydroxyl (–OH) groups at the 7th and 27th carbon positions of the cholesterol molecule. In biological systems, 7α,27-OHC acts as an important signaling molecule, particularly exhibiting significant biological activity in the immune system. It is also considered a ligand for several nuclear receptors and is involved in regulating physiological processes such as cholesterol metabolism and inflammatory responses.

 

By binding to its target receptors, 7α,27-OHC can influence the migration, function, and distribution of immune cells, thereby playing a key role in modulating immune responses. Due to its critical role in regulating immune cell function, 7α,27-OHC is considered a potential target for drug development. Quantitative analysis of 7α,27-OHC can help assess its level changes under different pathological conditions, assisting in validating drug targets associated with it and providing a basis for designing new immunomodulatory drugs. The production of 7α,27-OHC involves multiple enzymatic steps, particularly catalyzed by cholesterol 7α-hydroxylase (CYP7A1) and 27-hydroxylase (CYP27A1). Quantitative analysis of 7α,27-OHC and its metabolites can help reveal the activity of these metabolic pathways in different tissues, explore their changes under physiological and pathological conditions, and provide important insights for studying the mechanisms underlying metabolism-related diseases.

 



Figure 1. The Structure of 7α,27-Dihydroxycholesterol

 

MtoZ Biolabs extends its cutting-edge 7α,27-Dihydroxycholesterol Analysis Service, backed by our world-class liquid chromatography-mass spectrometry (LC-MS) platform and professional metabolomics team. We offer a comprehensive analysis of 7α,27-dihydroxycholesterol, promising high sensitivity and accuracy.

 

Analysis Workflow

 



 

Service Advantages

1. Continuously refined protocols and cutting-edge analytical tools  

 

2. Expert experimental planning  

 

3. Rapid processing times  

 

4. Exceptional precision, selectivity, and sensitivity

 

Applications

• Qualitative analysis of 7α,27-dihydroxycholesterol metabolites
• Quantitative analysis of 7α,27-dihydroxycholesterol content
• Facilitating drug discovery and development

 

Sample Submission Requirements

1. Sample Types

Serum, plasma, urine, tissues, and other biological samples. It is necessary to select more than three materials with the same condition for each sample. 

 

2. Sample Volume

• Animal tissue ≥ 100 mg
• Plant tissue ≥ 200 mg
• Serum/ Plasma ≥ 200 μL
• Urine ≥ 2 mL

 

3. Sample Preservation

Store at -80°C to maintain stability.

 

Note: Please provide details on sample collection and handling.

 

Deliverables

1. Experimental Procedures

2. Relevant Liquid Chromatography and Mass Spectrometry Parameters

3. Detailed Information on 7α,27-Dihydroxycholesterol

4. Raw Data

5. Custom Analysis Report

 

Our 7α,27-dihydroxycholesterol analysis service is designed to support your research and product development activities with precise and trustworthy data. We look forward to serving your specific requirements and contributing to your endeavors.