7α,25-Dihydroxycholesterol Analysis Service

7α,25-Dihydroxycholesterol (7α,25-OHC) is a dihydroxylated derivative of cholesterol, characterized by the presence of two hydroxyl (–OH) groups at the 7th and 25th carbon positions of the cholesterol molecule. In biological systems, 7α,25-OHC acts as a unique signaling molecule with significant biological activity, particularly within the immune system. It is an endogenous high-affinity ligand for the G protein-coupled receptor EBI2 (also known as GPR183). EBI2 is highly expressed in various immune cells, such as B cells and dendritic cells, and plays a critical role in regulating the migration, distribution, and function of these immune cells through its interaction with 7α,25-OHC. The critical role of 7α,25-OHC and its receptor EBI2 in modulating immune cell function makes it a potential target for drug development. Quantitative analysis of 7α,25-OHC can help evaluate its level changes under different pathological conditions, assisting in the validation of EBI2-related drug targets and providing a basis for designing new immunomodulatory drugs.

 

The biosynthesis of 7α,25-OHC involves multiple enzymatic steps, including those catalyzed by CYP7A1 and CYP27A1. Quantitative analysis of 7α,25-OHC and its metabolites can help reveal the activity of these metabolic pathways in different tissues, explore their changes under physiological and pathological conditions, and provide insights for studying the mechanisms underlying metabolism-related diseases.

 



Figure 1. The Structure of 7α,25-Dihydroxycholesterol

 

MtoZ Biolabs offers a top-tier 7α,25-Dihydroxycholesterol Analysis Service, supported by our high-quality liquid chromatography-mass spectrometry (LC-MS) platform and proficient metabolomics team. Our service delivers a thorough analysis of 7α,25-dihydroxycholesterol, providing high sensitivity and accuracy.

 

Analysis Workflow

 



 

Service Advantages

1. Continuously refined protocols and cutting-edge analytical tools

 

2. Expert experimental planning  

 

3. Rapid processing times  

 

4. Exceptional precision, selectivity, and sensitivity

 

Applications

• Qualitative analysis of the metabolites of 7α,25-dihydroxycholesterol
• Quantitative analysis of 7α,25-dihydroxycholesterol content
• Providing a foundation for improvement

 

Sample Submission Requirements

1. Sample Types

Serum, plasma, urine, tissues and other biological samples. It is necessary to select more than 3 materials with the same condition for each sample. 

 

2. Sample Volume

• Animal tissue ≥ 100 mg
• Plant tissue ≥ 200 mg
• Serum/ Plasma ≥ 200 μL
• Urine ≥ 2 mL

 

3. Sample Preservation

Samples should be stored at -80°C to maintain stability.

 

Note: Provide details on sample collection and handling.

 

Deliverables

1. Experimental Procedures

2. Relevant Liquid Chromatography and Mass Spectrometry Parameters

3. Detailed Information on 7α,25-Dihydroxycholesterol

4. Raw Data

5. Custom Analysis Report

 

Through our 7α,25-dihydroxycholesterol analysis service, we strive to support your research and product development processes by providing trustworthy and detailed results.