7,12-Diketolithocholic Acid Analysis Service

7,12-Diketolithocholic acid (7,12-DKLA) is a bile acid metabolite produced from the further oxidation of lithocholic acid (LCA). Its unique 7- and 12-keto groups impart distinct biological activities within the bile acid metabolism process. As a secondary bile acid, 7,12-DKLA is typically generated in the liver and intestines and is involved in various physiological processes, including bile acid synthesis, secretion, and metabolism. Research has shown that changes in 7,12-DKLA levels may be closely associated with hepatobiliary diseases such as cholestasis, cirrhosis, and gallstones. Additionally, 7,12-DKLA may play a significant role in metabolic disorders, including fatty liver disease, diabetes, and obesity. Given its potential significance in various pathological conditions, precise analysis of 7,12-DKLA is crucial not only for early diagnosis and monitoring of diseases but also for informing clinical treatment strategies.

 



Figure 1. Molecular Structure of 7,12-Diketolithocholic Acid

 

MtoZ Biolabs offers 7,12-Diketolithocholic Acid Analysis Service using high performance liquid chromatography-mass spectrometry (HPLC-MS). This advanced method combines the separation capabilities of HPLC with the detection power of MS, ensuring high sensitivity and specificity in 7,12-DKLCA detection.

 

Analysis Workflow

 



 

Service Advantages

1. High Sensitivity

Detects very low concentrations of 7,12-DKLCA accurately.

 

2. High Specificity

Differentiates 7,12-DKLCA from other compounds using mass spectrometry.

 

3. Rapid Analysis

Efficient sample preparation and fast detection processes for high-throughput analysis.

 

4. High Accuracy

Strict quality control ensures accuracy and reproducibility.

 

5. Versatility

Capable of analyzing 7,12-DKLCA and its related metabolites.

 

Sample Submission Requirements

1. Sample Types

Serum, plasma, urine, feces, tissue extracts, and other biological samples.

 

2. Sample Volume

At least 200 μL of liquid sample or 200 mg of solid sample.

 

3. Sample Preservation

Store at -80°C to maintain stability.

 

Note: Provide details on sample collection and handling.

 

Deliverables

1. Experimental Procedures

2. Relevant Liquid Chromatography and Mass Spectrometry Parameters

3. Detailed Information on 7,12-Diketolithocholic Acid

4. Raw Data

5. Custom Analysis Report

 

Our 7,12-Diketolithocholic Acid Analysis Service provides high-quality data support, advancing your scientific research and applications. For any inquiries or requirements, please contact us.