5S,12R-dihydroxy-6E,8E,10E,14Z-eicosatetraenoic Acid Analysis Service

5S,12R-dihydroxy-6E,8E,10E,14Z-eicosatetraenoic acid, abbreviated as 5S,12R-diHETE, is a polyunsaturated fatty acid metabolite. It is produced in the body from arachidonic acid via the lipoxygenase pathway, specifically through the action of 5-lipoxygenase and 12-lipoxygenase. Structurally, this lipid metabolite is characterized by four double bonds and two hydroxyl groups, which contribute to its physicochemical properties and biological functions. As a metabolite of arachidonic acid, 5S,12R-diHETE is involved in various physiological processes, including inflammation, cell proliferation, and vascular functions. Therefore, its analysis and quantification are of great interest in biomedical and clinical research, particularly in the context of inflammatory diseases, cancer, and cardiovascular disorders.

 

Given the involvement of 5S,12R-diHETE in numerous biological processes and pathologies, understanding its behavior, distribution and function can provide key insights into the pathogenesis and potential therapeutic strategies for various diseases. Its analysis can also be used as a biomarker for disease diagnosis and prognosis.

 



 

Figure 1. The Structure of 5S,12R-dihydroxy-6E,8E,10E,14Z-eicosatetraenoic Acid

 

MtoZ Biolabs provides a comprehensive 5S,12R-diHETE analysis service, using our state-of-the-art liquid chromatography-mass spectrometry (LC-MS) platform. We can help you:

• Detect and quantify 5S,12R-diHETE in various sample types, including biological fluids, cells, and tissues.
• Analyze the metabolic pathways of 5S,12R-diHETE.
• Investigate the role of 5S,12R-diHETE in different biological processes and diseases.

 

Analysis Workflow

 



 

Service Advantages

• Expertise in lipidomics and metabolomics: We have a team of experts with extensive experience in lipid and metabolite analysis.
• Advanced analytical platform: We use state-of-the-art LC-MS instruments for accurate and sensitive detection of 5S,12R-diHETE.
• Customizable service: We offer tailored analysis based on your specific research needs.

 

Applications

• Disease biomarker discovery and validation.
• Investigation of the role of 5S,12R-diHETE in disease pathogenesis.
• Therapeutic target identification and validation.

 

Sample Submission Requirements

1. Sample Types

Serum, plasma, urine, tissues, and other biological samples. It is recommended to prepare at least 3 biological replicates for each sample type.

 

2. Sample Volume

• Animal tissue ≥ 100 mg
• Plant tissue ≥ 200 mg
• Serum/ Plasma ≥ 200 μL
• Urine ≥ 2 mL

 

3. Storage and Shipping

Samples should be stored at -80℃ and shipped with dry ice. Avoid repeated cycles of freezing and thawing

 

Note: Please provide details on sample collection and handling.

 

Deliverables

1. Comprehensive Experimental details (Materials, Instruments, and Methods, etc.)

2. Relevant Liquid Chromatography and Mass Spectrometry Parameters

3. Detailed Information on 5S,12R-dihydroxy-6E,8E,10E,14Z-eicosatetraenoic Acid

4. Raw Data

5. Custom Analysis Report

 

Please feel free to contact us if you have any questions or need further information about our 5S,12R-dihydroxy-6E,8E,10E,14Z-eicosatetraenoic Acid Analysis Service. Our team at MtoZ Biolabs is ready to assist you in your research journey.