5α-Pregnane-3,20-Dione Analysis Service
- Uncovering potential disease biomarkers
- Enhancing disease diagnosis accuracy
- Facilitating the discovery of novel therapeutics
- Providing deeper insights into disease mechanisms
- Animal tissue ≥ 100 mg
- Plant tissue ≥ 200 mg
- Serum/ Plasma ≥ 200 μL
- Urine ≥ 2 mL
5α-Pregnane-3,20-dione (chemical formula C21H32O2) is a steroid compound with a structure composed of a steroid backbone made up of four carbon rings (A, B, C, D rings). The “5α” designation indicates that the hydrogen atom at the 5th carbon position is in the alpha configuration (below the molecular plane), giving it a specific spatial conformation. The molecule has two ketone groups (-C=O) at the 3rd and 20th carbon positions, which contribute to its unique biological activity. 5α-Pregnane-3,20-dione is a neuroactive steroid with sedative and anxiolytic properties. It interacts with γ-aminobutyric acid A (GABA_A) receptors, enhancing the inhibitory neurotransmitter effects of GABA, thus producing calming and anti-anxiety effects in the central nervous system. Additionally, 5α-pregnane-3,20-dione may play a role in regulating hormone balance, influencing the menstrual cycle and pregnancy maintenance. In the body, 5α-pregnane-3,20-dione is further metabolized into other steroid hormones, such as 5α-dihydroprogesterone. The metabolites of 5α-pregnane-3,20-dione also exhibit neuroactive properties, with effects similar to the parent compound in the central nervous system. These metabolites may regulate inhibitory signaling in the nervous system, providing sedative, anxiolytic, and sleep-regulating effects. Some of these metabolites also possess weak hormonal activity and may participate in the regulation of the reproductive system.
Analyzing the levels of 5α-pregnane-3,20-dione and its metabolites in biological samples is of great clinical and research significance. In humans, the level of 5α-pregnane-3,20-dione can reflect the state of progesterone metabolism, and it has potential diagnostic value in studies related to neurological regulation, hormone balance, and reproductive health. Additionally, it plays an important role in research on neurological disorders, anxiety, and menstrual irregularities. Using mass spectrometry and other highly sensitive analytical techniques allows for precise detection of 5α-pregnane-3,20-dione and its metabolites in biological samples. This is crucial for advancing the understanding of steroid hormone metabolism, developing related medications, and monitoring hormone metabolism-related disorders.
Figure 1. The Structure of 5α-Pregnane-3,20-Dione
MtoZ Biolabs, an integrated chromatography and mass spectrometry (MS) services provider, providing advanced metabolomics, proteomics and biopharmaceutical analysis services to researchers in biochemistry, biotechnology, and biopharmaceutical fields. Our ultimate aim is to provide more rapid, high-throughput, and cost-effective analysis, with exceptional data quality and minimal sample consumption. MtoZ Biolabs utilizes an LC-MS-based metabolomics research platform for the analysis of 5α-pregnane-3,20-dione. We ensure the accuracy of the quantification of 5α-pregnane-3,20-dione and its metabolites, as well as the comprehensiveness and precision of metabolite identification. If you are interested in our 5α-pregnane-3,20-dione analysis service, please feel free to contact us at any time, and we will be fully committed to providing you with high-quality analytical services and technical support.
Analysis Workflow
Service Advantages
1. Advance Analysis Platform: MtoZ Biolabs established an advanced 5α-pregnane-3,20-dione analysis platform, guaranteeing reliable, fast, and highly accurate analysis service.
2. One-Time-Charge: Our pricing is transparent, no hidden fees or additional costs.
3. High-Data-Quality: Extensive data coverage with rigorous quality control. Our advanced analysis platform consolidates all 5α-pregnane-3,20-dione analysis data, delivering clients a detailed and comprehensive data report.
4. Quick Turnaround: We offer a fast analysis process to ensure that clients receive high-quality data within a short time.
Applications
Sample Submission Requirements
1. Sample Types
Serum, plasma, urine, tissues, and other biological samples. Inclusion of at least three samples under the same condition is required for each type.
2. Sample Volume
3. Sample Preservation
Samples should be stored at -80°C to maintain stability.
Note: Please provide detailed information on sample collection and handling procedures.
Deliverables
1. Experimental Procedures
2. Relevant Liquid Chromatography and Mass Spectrometry Parameters
3. Detailed Information on 5α-Pregnane-3,20-Dione
4. Raw Data
5. Custom Analysis Report
Our 5α-Pregnane-3,20-Dione Analysis Service is designed to support your research and product development efforts by delivering accurate and reliable results.
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