5-methyluridine Analysis Service

5-methyluridine (m5U) is a pyrimidine nucleoside, featuring a methyl group attached to the 5-position of the uracil base.  It is one of the most common modifications found in cellular RNA. m5U is almost universally present at position 54 in eukaryotic and bacterial tRNA, where it plays a crucial role in stabilizing the structure of these molecules. The same “T loop” motif is also observed in various other forms of non-coding RNA, including tmRNA and rRNA.

Research has shown that the absence of tRNA modifications like m5U typically does not lead to drastically different phenotypes. However, understanding the nuances of 5-methyluridine and its modifications is essential for deciphering RNA biology and its impact on cellular processes, such as translation and stress responses. Given its significance, m5U is emerging as a potential biomarker for various diseases, including cancer.

Figure 1. The Structure of 5-methyluridine

At MtoZ Biolabs, we are dedicated to providing accurate, reliable, and affordable 5-methyluridine analysis service, employing state-of-the-art Mass Spectrometry techniques, supported by our team's solid expertise in metabolomics. We help our clients to study the roles of 5-methyluridine in various biological processes, understand the pathogenesis of diseases, and explore potential therapeutics.

Analysis Workflow

Service Advantages

• State-of-the-art LC-MS/MS platforms allow us to accurately and reliably quantify 5-methyluridine in various types of samples.
• A team of experts in mass spectrometry and metabolomics provides professional data analysis and interpretation.
• One-stop service from sample preparation, LC-MS/MS analysis to data analysis, saving your time and efforts.
• Competitive price with high-quality service.

Applications

Our 5-methyluridine analysis service can be applied in various fields, including but not limited to:

• Disease Biomarker Discovery
• Drug Development
• Clinical Research

Sample Submission Requirements

1. Sample Types

Serum, plasma, urine, tissues, and other biological samples. It is recommended to prepare at least 3 biological replicates for each sample type.

2. Sample Volume

• Animal tissue ≥ 100 mg
• Plant tissue ≥ 200 mg
• Serum/ Plasma ≥ 200 μL
• Urine ≥ 2 mL

3. Storage and Shipping

Samples should be stored at -80℃ and shipped with dry ice. Avoid repeated cycles of freezing and thawing

Note: Please provide details on sample collection and handling.

Deliverables

1. Experimental Procedures

2. Relevant Liquid Chromatography and Mass Spectrometry Parameters

3. Detailed Information on 5-methyluridine

4. Raw Data

5. Custom Analysis Report

To learn more about our 5-methyluridine analysis service, please contact us at MtoZ Biolabs. We are looking forward to assisting you with your research needs.