5-Methylcytosine Analysis Service
- Extensive experience in various biological sample preparation techniques
- Professional standard operating procedure
- State-of-the-art facilities and equipment for precise analysis
- Expert team with extensive experience in metabolomics
- Comprehensive data analysis
- Excellent customer service and after-service support
- Uncovering epigenetic modifications in DNA
- Identifying biomarkers for cancer research
- Enhancing understanding of gene expression regulation
- Expedite drug discovery process
- DNA ≥ 2 μg
- Cell lines ≥ 1× 10⁶ cells
- Tissue ≥ 50 mg
5-Methylcytosine (5mC) is an important biomolecule and a form of DNA modification, with the chemical formula C₄H₅N₃O. It is formed through the DNA methylation of cytosine, typically occurring at the cytosine residues of CpG dinucleotides (CpG dinucleotide refers to a DNA sequence composed of one cytosine (C) and one guanine (G), with a phosphodiester bond connecting the C and G). 5-Methylcytosine plays a crucial role in regulating gene expression, cell differentiation, and development. It is often associated with transcriptional repression by recruiting repressive factors or altering chromatin conformation to inhibit gene expression. Analyzing the levels of 5-methylcytosine can reveal its role in gene silencing and transcriptional regulation, aiding in the understanding of the complex mechanisms of gene expression. Additionally, examining the levels of 5-methylcytosine and its metabolites supports the implementation of personalized medicine by providing biomarkers for therapeutic intervention.
Figure 1. The Structure of 5-Methylcytosine
MtoZ Biolabs offers a comprehensive 5-Methylcytosine Analysis Service, underpinned by our state-of-the-art liquid chromatography-mass spectrometry (LC-MS) platform and experienced metabolomics team. Our service ensures high sensitivity and accuracy in the analysis of 5-methylcytosine.
Analysis Workflow
Service Advantages
Applications
Sample Submission Requirements
1. Sample Types
DNA, cell lines, tissues, and other biological samples. It is necessary to select more than 3 materials with the same condition for each sample.
2. Sample Volume
3. Sample Preservation
Store at -80°C to maintain stability.
Note: Provide details on sample collection and handling.
Deliverables
1. Experimental Procedures
2. Relevant Liquid Chromatography and Mass Spectrometry Parameters
3. Detailed Information on 5-Methylcytosine
4. Raw Data
5. Custom Analysis Report
Through our 5-methylcytosine analysis service, we can help you unveil the mysteries of epigenetic modification and expedite your research and development process.
How to order?
