5-methylcytidine Analysis Service
- State-of-the-art LC-MS/MS platforms allow us to accurately and reliably quantify m5C in various types of samples.
- A team of experts in mass spectrometry and metabolomics provide professional data analysis and interpretation.
- One-stop service from sample preparation, LC-MS/MS analysis to data analysis, saving your time and efforts.
- Competitive price with high-quality service.
- Biomedical research: To understand the role of m5C in biological processes and diseases
- Drug discovery: To identify potential therapeutic targets related to m5C modification
- Clinical diagnostics: To develop m5C-based biomarkers for disease diagnosis and prognosis
- Animal tissue ≥ 100 mg
- Plant tissue ≥ 200 mg
- Serum/ Plasma ≥ 200 μL
- Urine ≥ 2 mL
5-methylcytidine (m5C) is a significant component of RNA found in various organisms, including Escherichia coli, plants, and animals. This modification is integral to the DNA methylation processes studied in epigenetics, where it influences gene expression and cellular identity. 5-methylcytidine is a methylated pyrimidine that enhances stacking interactions within nucleic acids, contributing to increased thermal stability of RNA structures. This property is crucial for maintaining the structural integrity of RNA during various biological processes, including translation and gene regulation. Changes in the levels of m5C can influence gene expression patterns, contributing to developmental processes and disease mechanisms, including cancer. Research indicates that alterations in m5C levels may serve as biomarkers for certain conditions, providing insights into cellular state and potential therapeutic targets.
Figure 1. The Structure of 5-methylcytidine
Given its critical role in various biological processes and disease mechanisms, a comprehensive understanding of m5C modification in RNA is essential. This can help in identifying therapeutic targets and developing effective treatments for diseases associated with m5C modification. Additionally, m5C can also be used as a potential biomarker for disease diagnosis and prognosis.
At MtoZ Biolabs, we offer high-quality 5-methylcytidine analysis service, using advanced LC-MS/MS technology. With our extensive experience in metabolomics and advanced analytical platform, we can ensure accurate, reliable, and reproducible results.
Analysis Workflow
Service Advantages
Applications
Our m5C analysis service can be applied in various fields such as:
Sample Submission Requirements
1. Sample Types
Serum, plasma, urine, tissues, and other biological samples. It is recommended to prepare at least 3 biological replicates for each sample type.
2. Sample Volume
3. Storage and Shipping
Samples should be stored at -80℃ and shipped with dry ice. Avoid repeated cycles of freezing and thawing
Note: Please provide details on sample collection and handling.
Deliverables
1. Experimental Procedures
2. Relevant Liquid Chromatography and Mass Spectrometry Parameters
3. Detailed Information on 5-methylcytidine
4. Raw Data
5. Custom Analysis Report
At MtoZ Biolabs, we are committed to providing high-quality services to meet your research needs. Contact us today to discuss your project requirements.
How to order?
