5-methoxycarbonylmethyluridine Analysis Service

5-methoxycarbonylmethyluridine (mcm5U) is a modified nucleoside that features a methoxycarbonylmethyl group attached to the 5th carbon of the uracil base. This unique structural modification is predominantly found in certain types of RNA, including transfer RNA (tRNA). The incorporation of mcm5U into tRNA can improve codon-anticodon recognition and enhance the overall efficiency of protein synthesis, particularly under varying cellular conditions. By contributing to the structural integrity of tRNA, this modification helps ensure accurate translation, which is vital for maintaining cellular function and responding to environmental stresses.

 



 

Figure 1. The Structure of 5-methoxycarbonylmethyluridine

 

Alterations in RNA modifications, including 5-methoxycarbonylmethyluridine, have been associated with various biological processes, including development, cellular stress responses, and disease mechanisms. Understanding the presence and function of 5-methoxycarbonylmethyluridine in biological systems could provide valuable insights into its potential as a biomarker for disease and its implications in therapeutic development.

 

At MtoZ Biolabs, we provide accurate and reliable analysis of mcm5U, aiding in advancing your research. Our sophisticated mass spectrometry platforms and competent technical team are able to detect and quantify mcm5U even at trace levels.

 

Analysis Workflow

 



 

Service Advantages

• State-of-the-art LC-MS/MS platforms allow us to accurately and reliably quantify mcm5U in various types of samples.
• A team of experts in mass spectrometry and metabolomics provides professional data analysis and interpretation.
• One-stop service from sample preparation, LC-MS/MS analysis to data analysis, saving your time and efforts.
• Competitive price with high-quality service.

 

Applications

Our mcm5U analysis service serves a wide range of fields, including but not limited to:

• Biomarker discovery
• Therapeutic target identification
• Pathway analysis
• Drug efficacy studies

 

Sample Submission Requirements

1. Sample Types

Serum, plasma, urine, tissues, and other biological samples. It is recommended to prepare at least 3 biological replicates for each sample type.

 

2. Sample Volume

• Animal tissue ≥ 100 mg
• Plant tissue ≥ 200 mg
• Serum/ Plasma ≥ 200 μL
• Urine ≥ 2 mL

 

3. Storage and Shipping

Samples should be stored at -80℃ and shipped with dry ice. Avoid repeated cycles of freezing and thawing

 

Note: Please provide details on sample collection and handling.

 

Deliverables

1. Experimental Procedures

2. Relevant Liquid Chromatography and Mass Spectrometry Parameters

3. Detailed Information on 5-methoxycarbonylmethyluridine

4. Raw Data

5. Custom Analysis Report

 

For more details on our 5-methoxycarbonylmethyluridine analysis service, please feel free to contact us. At MtoZ Biolabs, we are committed to providing comprehensive solutions to advance your research.