5-hydroxymethylcytidine Analysis Service

5-hydroxymethylcytidine (hm5C) is a derivative of cytidine, found in the DNA of cells. It is an offspring of 5-methylcytidine (5mC), which is a product of an enzymatically controlled process of DNA methylation. The occurrence of hm5C is a significant epigenetic modification that is pervasively present in human tissues, especially abundant in the brain and embryonic stem cells. hm5C plays a critical role in a myriad of biological processes, such as gene expression, DNA methylation dynamics, and embryonic development. Moreover, abnormal hm5C levels have been associated with various pathologies, including cancer, neurological disorders, and autoimmune diseases.

 



 

Figure 1. The Structure of 5-hydroxymethylcytidine

 

The understanding of how hm5C fits into the epigenetic landscape is still in its infancy. It's essential to develop an accurate, sensitive, and high-throughput method for the quantitative analysis of hm5C to garner a better understanding of its biological function and significance in health and disease.

 

At MtoZ Biolabs, we utilize LC-MS/MS for the analysis of hm5C, offering quantification for hm5C and its derivatives. Our well-established method allows us to detect hm5C in a broad range of biological samples, providing accurate, reliable, and rapid results.

 

Analysis Workflow

 



 

Service Advantages

• State-of-the-art LC-MS/MS platforms allow us to accurately and reliably quantify 5-hydroxymethylcytidine in various types of samples.
• A team of experts in mass spectrometry and metabolomics provides professional data analysis and interpretation.
• One-stop service from sample preparation, LC-MS/MS analysis to data analysis, saving your time and efforts.
• Competitive price with high-quality service.

 

Applications

This service is useful for the study of epigenetic modifications, DNA methylation dynamics, and disease pathogenesis.

 

Sample Submission Requirements

1. Sample Types

Serum, plasma, urine, tissues, and other biological samples. It is recommended to prepare at least 3 biological replicates for each sample type.

 

2. Sample Volume

• Animal tissue ≥ 100 mg
• Plant tissue ≥ 200 mg
• Serum/ Plasma ≥ 200 μL
• Urine ≥ 2 mL

 

3. Storage and Shipping

Samples should be stored at -80℃ and shipped with dry ice. Avoid repeated cycles of freezing and thawing

 

Note: Please provide details on sample collection and handling.

 

Deliverables

1. Experimental Procedures

2. Relevant Liquid Chromatography and Mass Spectrometry Parameters

3. Detailed Information on 5-hydroxymethylcytidine

4. Raw Data

5. Custom Analysis Report

 

Through our 5-hydroxymethylcytidine Analysis Service, MtoZ Biolabs is dedicated to pushing forward the frontier of epigenetics research, revealing the mysteries of hm5C, and assisting our clients in their quest for scientific breakthroughs.