5-formylcytidine Analysis Service

5-formylcytidine is a modified pyrimidine nucleoside. 5-formylcytidine is not only a stable epigenetic mark but also an intermediate in active DNA demethylation. It regulates gene expression, and has been shown to play critical roles in embryonic stem cell self-renewal and differentiation. 5-formylcytidine also participates in various biological processes, including transcription regulation and DNA damage repair. Its dysregulation is associated with various diseases, including cancer. Therefore, studying 5-formylcytidine is crucial for understanding its biological functions and potential clinical applications.

 



 

Figure 1. The Structure of 5-formylcytidine

 

With the development of high-resolution mass spectrometry, the detection and quantification of 5-formylcytidine in complex biological samples have become possible. However, due to the low abundance and instability of 5-formylcytidine, the reliable detection and quantification of 5-formylcytidine remain challenging.

 

At MtoZ Biolabs, we provide a comprehensive 5-formylcytidine analysis service using high-performance liquid chromatography coupled with tandem mass spectrometry (HPLC-MS/MS), which offers high sensitivity and accuracy. Our experienced scientists will work closely with you to customize the best solution for your research.

 

Analysis Workflow

 



 

Service Advantages

• Extensive expertise in handling various biological samples and extracting metabolites.
• State-of-the-art mass spectrometry platforms: HPLC-MS, GC-MS, MRM method.
• Strict quality control to ensure reliable results.
• Comprehensive statistical and bioinformatics analysis.
• Personalized service to meet various research needs.

 

Applications

The 5-formylcytidine analysis service is applicable in many areas, such as:

• Understanding the role of 5-formylcytidine in gene regulation and embryonic development.
• Investigating the relationship between 5-formylcytidine levels and various diseases.
• Developing potential biomarkers for disease diagnosis and prognosis.

 

Sample Submission Requirements

 



 

• Samples should be stored at -80℃ and shipped with dry ice.
• We recommend providing at least 3 samples of similar conditions for each type of sample.
• Please provide details on sample collection and handling.
• Other sample types, please contact us for more details.

 

Deliverables

1. Experimental Procedures

2. Relevant Liquid Chromatography and Mass Spectrometry Parameters

3. Detailed Information on 5-formylcytidine

4. Raw Data

5. Custom Analysis Report

 

Please don't hesitate to contact us if you have any questions about our 5-formylcytidine analysis service. At MtoZ Biolabs, we are dedicated to providing the most reliable and precise metabolomics analysis service for our customers.