5-carboxycytidine Analysis Service

5-carboxycytidine is a modification of cytidine, a constituent of nucleic acids, predominantly found in eukaryotic organisms. Structurally, it is distinguished from cytidine by the presence of a carboxyl group on the fifth carbon atom, hence the name 5-carboxycytidine. 5-carboxycytidine is one of the oxidation products of 5-methylcytosine (5mC), produced via the ten-eleven translocation (TET) family of enzymes. It acts as an intermediate in the demethylation process of DNA, critical for epigenetic regulation of gene expression, cellular development, and differentiation, as well as tumorigenesis.

 



 

Figure 1. The Structure of 5-carboxycytidine

 

The study of 5-carboxycytidine is of paramount importance, given its role in the demethylation process and its potential implications in various diseases, including cancer. The ability to accurately quantitate 5-carboxycytidine in biological samples, therefore, provides a robust tool for research in epigenetic regulation and disease pathologies.

 

At MtoZ Biolabs, we offer a comprehensive 5-carboxycytidine analysis service, utilizing advanced ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS), to provide accurate quantitation and profiling of 5-carboxycytidine.

 

Analysis Workflow

 



 

Service Advantages

1. Continuously refined protocols and cutting-edge analytical tools  

2. Expert experimental planning  

3. Rapid processing times  

4. Exceptional precision, selectivity, and sensitivity 

 

Applications

Our 5-carboxycytidine analysis service can be applied in various fields, including but not limited to:

• Epigenetic research
• Disease pathologies, in particular, cancer research
• Drug development and pharmacokinetics

 

Sample Submission Requirements

 



 

• Samples should be stored at -80℃ and shipped with dry ice.
• We recommend providing at least 3 samples of similar conditions for each type of sample.
• Please provide details on sample collection and handling.
• Other sample types, please contact us for more details.

 

Deliverables

1. Experimental Procedures

2. Relevant Liquid Chromatography and Mass Spectrometry Parameters

3. Detailed Information on 5-carboxycytidine

4. Raw Data

5. Custom Analysis Report

 

At MtoZ Biolabs, we are committed to providing you with comprehensive metabolomics services. Our rigorous quality control and analytical process ensure to provide reliable results to accelerate your research.