5β-Androsterone Analysis Service | Steroid hormones

5β-Androsterone is a metabolite of androgens, specifically a breakdown product of testosterone and dihydrotestosterone (DHT). It is a steroid hormone that belongs to the family of 17-ketosteroids and is characterized by a 5β configuration, where the hydrogen atom at the 5th position is in a β-orientation. This structural feature distinguishes it from its 5α isomer, 5α-androsterone, and affects its biological activity and metabolism. 5β-Androsterone itself is a less active form of androgen and is often considered a marker of androgen metabolism. It is primarily produced in the liver through the action of 5β-reductase enzymes, which reduce testosterone and other androgens. 5β-Androsterone is then conjugated to glucuronic acid or sulfate to form water-soluble derivatives, which are excreted in urine.

Metabolomics analysis of 5β-androsterone utilizes high-throughput techniques such as mass spectrometry and nuclear magnetic resonance to comprehensively study the dynamic changes and metabolic pathways of 5β-androsterone and its metabolites within the body. This analytical approach is essential for elucidating the biological functions of 5β-androsterone , optimizing its clinical applications, evaluating the effects of its deficiency or excess on health, and promoting personalized medical interventions.

Figure 1. The Structure of 5β-Androsterone

MtoZ Biolabs introduces the cutting-edge 5β-Androsterone Analysis Service, backed by our state-of-the-art liquid chromatography-mass spectrometry (LC-MS) platform and professional metabolomics team. Our service provides a comprehensive 5β-androsterone analysis, ensuring high sensitivity and accuracy.

Analysis Workflow

Service Advantages

1. High quality data: We deliver reliable and reproducible results with our advanced LC-MS system.

2. Expertise: Our experienced team of scientists ensures the quality and accuracy of our analysis.

3. Comprehensive report: We provide a detailed report including the experimental procedure, instrument parameters, and results interpretation.

4. Fast turnaround: The process from sample handling to report generation is efficient, shortening the analysis cycle.

Applications

• Qualitative and quantitative analysis of 5β-androsterone
• Investigating the role of 5β-androsterone in various biological processes
• Monitoring changes in 5β-androsterone levels under different conditions

Sample Submission Requirements

1. Sample Types

Serum, plasma, urine, tissues and other biological samples. For each sample, it is necessary to select more than 3 materials under the same condition.

2. Sample Volume

• Animal tissue ≥ 100 mg
• Plant tissue ≥ 200 mg
• Serum/ Plasma ≥ 200 μL
• Urine ≥ 2 mL

3. Sample Preservation

Store at -80°C to maintain stability.

Note: Please provide details on sample collection and handling.

Deliverables

1. Experimental Procedures

2. Relevant Liquid Chromatography and Mass Spectrometry Parameters

3. Detailed Information on 5β-Androsterone

4. Raw Data

5. Custom Analysis Report

Our 5β-androsterone analysis service is designed to support your research and product development by delivering accurate and reliable results.