5,2'-O-dimethylcytidine Analysis Service

5,2'-O-Dimethylcytidine is a modified nucleoside that plays a significant role in the regulation of RNA stability and function. This compound features two methyl groups: one at the 5th position of the cytosine base and another at the 2'-hydroxyl group of the ribose sugar. These modifications enhance the stability of RNA molecules, particularly in transfer RNA (tRNA), and are crucial for proper cellular function and gene expression regulation.

 



 

Figure 1. The Structure of 5,2'-O-dimethylcytidine


This modified nucleoside, such as 5,2'-O-Dimethylcytidine, is found in various tRNA molecules and is essential for:


1. Enhancing RNA Stability: By reducing the susceptibility of RNA to degradation, it ensures the stability and longevity of the RNA in the cellular environment.


2. Regulating Gene Expression: The modification influences the interaction between RNA and proteins, affecting translation efficiency and gene regulation.


3. Understanding Epigenetic Modifications: 5,2'-O-dimethylcytidine serves as a marker for studying RNA modifications and their impacts on cellular processes and disease mechanisms.

 

MtoZ Biolabs offers a specialized 5,2'-O-Dimethylcytidine Analysis Service utilizing advanced mass spectrometry techniques. Our service provides precise quantification and detailed analysis of this modified nucleoside, enabling researchers to explore its role in RNA biology and its implications for health and disease.

 

Analysis Workflow

 



 

Service Advantages

• Advanced MS platform
• Highly sensitive and reliable analysis
• Comprehensive report with detailed results and professional interpretation
• Rapid turnaround time
• Excellent customer support

 

Applications

• Biomarker discovery
• Drug research
• Disease diagnosis
• RNA modification studies

 

Sample Submission Requirements

1. Sample Types

Serum, plasma, urine, tissues, and other biological samples. It is necessary to prepare more than 3 biological replicates for each type of sample.

 

2. Sample Volume

• Animal tissue ≥ 100 mg
• Plant tissue ≥ 200 mg
• Serum/ Plasma ≥ 200 μL
• Urine ≥ 2 mL

 

3. Storage and Shipping

Samples should be stored at -80℃ and shipped with dry ice.

 

Note: Please provide details on sample collection and handling.

 

Deliverables

1. Experimental Procedures

2. Relevant Liquid Chromatography and Mass Spectrometry Parameters

3. Detailed Information on 5,2'-O-dimethylcytidine

4. Raw Data

5. Custom Analysis Report

 

At MtoZ Biolabs, we are dedicated to providing you with the most reliable and accurate 5,2'-O-dimethylcytidine Analysis Service. For more information or to request a quote, please contact us.