17-α-Hydroxypregnenolone Analysis Service

17-α-Hydroxypregnenolone is a key steroid hormone and an essential intermediate in the steroidogenesis pathway. It is synthesized by the adrenal cortex and gonads and acts as a precursor to dehydroepiandrosterone (DHEA) and 17-hydroxyprogesterone, both of which play critical roles in various physiological processes. In the body, 17-α-hydroxypregnenolone is generated through the action of the mitochondrial enzyme 17α-hydroxylase (CYP17A1), found in the adrenal glands and gonads. Abnormal levels of this hormone are often linked to endocrine disorders, such as congenital adrenal hyperplasia (CAH), highlighting its importance in assessing adrenal function and diagnosing related conditions. Furthermore, 17-α-hydroxypregnenolone is valuable in research focused on hormone metabolism and the development of related pharmaceuticals.

Figure 1. Molecular Structure of 17-α-Hydroxypregnenolone

MtoZ Biolabs offers 17-α-Hydroxypregnenolone Analysis Service using high performance liquid chromatography-mass spectrometry (HPLC-MS). This advanced method combines the separation capabilities of HPLC with the detection power of MS, ensuring high sensitivity and specificity in 17-α-hydroxypregnenolone detection.

Analysis Workflow

Service Advantages

1. High Sensitivity and Precision

HPLC-MS/MS provides highly sensitive and accurate measurements, suitable for detecting low concentrations of 17-α-hydroxypregnenolone.

2. Rapid and Efficient

Optimized sample preparation and fast analysis ensure quick and efficient processing.

3. Wide Applicability

Suitable for various biological samples, including blood, urine, and tissues.

4. Accurate and Reliable Data

Ensures high reproducibility and low error rates, supporting both research and clinical applications, providing reliable and specific results.

Sample Submission Requirements

For detailed sample submission instructions, please consult our technical team.

Applications

• Essential for evaluating adrenal function, particularly in diagnosing CAH and other endocrine disorders.
• Supports investigations into the role of 17-α-hydroxypregnenolone in steroidogenesis.
• Used in studies of drug metabolism and pharmacodynamics related to 17-α-hydroxypregnenolone, aiding in drug development.
• Monitors 17-α-hydroxypregnenolone levels during treatment to guide clinical interventions.

Deliverables

1. Experimental Procedures

2. Relevant Liquid Chromatography and Mass Spectrometry Parameters

3. Detailed Information on 17-α-Hydroxypregnenolone

4. Raw Data

5. Custom Analysis Report

Our 17-α-Hydroxypregnenolone Analysis Service provides high-quality data support, advancing your scientific research and applications. For any inquiries or requirements, please contact us.