15S-hydroxy,14R-(S-cysteinyl)-5Z,8Z,10E,12E-eicosatetraenoic Acid Analysis Service

15S-hydroxy,14R-(S-cysteinyl)-5Z,8Z,10E,12E-eicosatetraenoic acid, also known as Eoxin E4 (EXE4) or 14,15-leukotriene E4, is a bioactive lipid mediator. Eoxins are produced through the metabolism of arachidonic acid by 15-lipoxygenase, forming 15(S)-hydroperoxyeicosapentaenoic acid (15(S)-HpETE). This precursor is subsequently converted into a series of intermediates, including EXA4, EXC4, and EXD4, which are finally transformed into EXE4 through the action of LTC4 synthase. This biosynthetic pathway closely mirrors the leukotriene formation pathway. EXA4 serves as a transient, intracellular intermediate that is quickly metabolized into downstream eoxins. These downstream metabolites, including EXE4, are secreted from their parent cells, where they are believed to play a regulatory role in allergic responses and the progression of certain cancers. While the full scope of their physiological functions is still being studied, EXE4 is considered a key player in the modulation of inflammatory and immune responses.

 



 

Figure 1. The Structure of 15S-hydroxy,14R-(S-cysteinyl)-5Z,8Z,10E,12E-eicosatetraenoic Acid

 

Given the emerging significance of EXE4 in the context of inflammation, allergic conditions, and cancer, its accurate quantification is essential for advancing research in these areas. Monitoring EXE4 levels provides valuable insights into its role in pathological conditions and may contribute to the development of novel therapeutic approaches.

 

MtoZ Biolabs is an industry-leading provider of comprehensive metabolomics services using state-of-the-art technology. One of our specialized services is 15S-hydroxy,14R-(S-cysteinyl)-5Z,8Z,10E,12E-eicosatetraenoic Acid Analysis Service, designed to provide a deep understanding of this metabolite for your research and development efforts.

• We provide reliable and accurate quantification of 15S-hydroxy,14R-(S-cysteinyl)-5Z,8Z,10E,12E-eicosatetraenoic Acid using advanced liquid chromatography-mass spectrometry (LC-MS) technologies.
• Our experienced team of scientists can assist in experimental design, data interpretation, and follow-up experiments.
• We offer a complete solution from sample preparation to detailed report delivery providing you with actionable insights for your research or discovery programs.

 

Analysis Workflow

 



 

Service Advantages

1. Advance Analysis Platform

MtoZ Biolabs established an advanced 15S-hydroxy,14R-(S-cysteinyl)-5Z,8Z,10E,12E-eicosatetraenoic acid analysis service platform, guaranteeing reliable, fast, and highly accurate analysis service.

 

2. One-Time-Charge

Our pricing is transparent, no hidden fees or additional costs.

 

3. High-Data-Quality

Deep data coverage with strict data quality control. AI-powered bioinformatics platform integrate all 15S-hydroxy,14R-(S-cysteinyl)-5Z,8Z,10E,12E-eicosatetraenoic acid analysis service data providing clients with a comprehensive data report.

 

4. Fast Turnaround

The process from sample handling to report generation is efficient, shortening the analysis cycle.

 

Applications

• Biomarker Discovery
• Drug Metabolism Studies
• Nutritional and Environmental Impact Studies

 

Sample Submission Requirements

1. Sample Types

Serum, plasma, urine, tissues, and other biological samples. It is recommended to prepare at least 3 biological replicates for each sample type.

 

2. Sample Volume

• Animal tissue ≥ 100 mg
• Plant tissue ≥ 200 mg
• Serum/ Plasma ≥ 200 μL
• Urine ≥ 2 mL

 

3. Storage and Shipping

Samples should be stored at -80℃ and shipped with dry ice. Avoid repeated cycles of freezing and thawing

 

Note: Please provide details on sample collection and handling.

 

Deliverables

1. Comprehensive Experimental details (Materials, Instruments, and Methods, etc.)

2. Relevant Liquid Chromatography and Mass Spectrometry Parameters

3. Detailed Information on 15S-hydroxy,14R-(S-cysteinyl)-5Z,8Z,10E,12E-eicosatetraenoic Acid

4. Raw Data

5. Custom Analysis Report

 

At MtoZ Biolabs, we are committed to providing the most reliable and highest quality services to our customers. Our 15S-hydroxy,14R-(S-cysteinyl)-5Z,8Z,10E,12E-eicosatetraenoic Acid Analysis Service can provide the deep insights you need to drive your research forward. Contact us today to learn more about how we can assist you in your project.