12-Ketolithocholic Acid Analysis Service

12-Ketolithocholic acid (12-KLCA) is a secondary bile acid derived from primary bile acids through microbial action in the intestine. Its distinctive 12-keto group sets it apart in terms of chemical properties and biological functions. 12-KLCA is primarily produced in the kidneys and has been suggested as a potential biomarker for kidney injury due to its significant role in renal damage. Furthermore, 12-KLCA plays key roles in cholesterol metabolism, liver function, and gut microbiota regulation. Changes in its metabolism are closely linked to the development of certain liver and intestinal diseases. Therefore, accurately analyzing the metabolic dynamics of 12-KLCA is crucial for understanding its function in health and disease.

 

Figure 1. Molecular Structure of 12-Ketolithocholic Acid

 

MtoZ Biolabs offers 12-Ketolithocholic Acid Analysis Service using high performance liquid chromatography-mass spectrometry (HPLC-MS). This advanced method combines the separation capabilities of HPLC with the detection power of MS, ensuring high sensitivity and specificity in 12-KLCA detection.

 

Analysis Workflow

 



 

Service Advantages

1. High Sensitivity

Detects very low concentrations of 12-KLCA accurately.

 

2. High Specificity

Differentiates 12-KLCA from other compounds using mass spectrometry.

 

3. Rapid Analysis

Efficient sample preparation and fast detection processes for high-throughput analysis.

 

4. High Accuracy

Strict quality control ensures accuracy and reproducibility.

 

5. Versatility

Capable of analyzing 12-KLCA and its related metabolites.

 

Sample Submission Requirements

 



For detailed sample submission instructions, please consult our technical team.

 

Deliverables

1. Experimental Procedures

2. Relevant Liquid Chromatography and Mass Spectrometry Parameters

3. Detailed Information on 12-Ketolithocholic Acid

4. Raw Data

5. Custom Analysis Report

 

MtoZ Biolabs is dedicated to advancing your research and clinical projects by providing detailed insights into 12-KLCA's extensive physiological roles through top-tier analytical techniques and expert services.